In an effort to re-establish tissue with odontogenic potential in the pulp space of immature permanent teeth, stimulated human dental pulp stem/progenitor cells (hDPSCs) have shown potential to differentiate and form mineralized matrix, marked by high expression of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN). Bacterial by-products have been shown to adversely affect cell differentiation. This study investigated the effect of P. gingivalis, a putative endodontic pathogen, and blockage of its host recognition on hDPSCs. Stimulated hDPSCs were exposed to varying concentrations of P. gingivalis by-product and gene expression of DSPP and OCN was measured. Cells were exposed to TLR2 blocking agents prior to exposure to the by-product. P. gingivalis affected dose-dependent suppression of the measured gene expression. Blockade of TLR2 inhibited the by-product derived suppression of gene expression. The immune-potential of by-product was confirmed to be detrimental to the differentiation of hDPSCs, and this effect could be moderated by TLR2-blockade.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/31618 |
| Date | 02 January 2012 |
| Creators | Tom-Kun Yamagishi, Valerie |
| Contributors | Glogauer, Michael |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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