Two experiments were conducted to investigate the effects of free Met and Lys supplementation on ruminal fermentation in vitro and in vivo. In the in vitro study, supplementation of Met and Lys had effects on concentrations of total VFA and NH<sub>4</sub><sup>+</sup>, proportions of acetate, propionate, butyrate, isovalerate, valerate, and the ratio of acetate to propionate. Percentages of microbial N synthesis in effluent pellets were not affected by supplementing Lys and Met. The combination of 0.52% Met and 1.03% (90% DM) Lys resulted in the highest concentration of total VFA, second highest concentration of NH<sub>4</sub><sup>+</sup>, and lowest ratio of acetate to propionate with no decrease in the microbial CP production. In the in vivo study, supplementing 0.29% Met and 2.27% Lys (100% DM) had no impact on mean DMI, OMI, milk yield, milk component production or percentage, SCCS, 4% FCM and ECM production efficiencies, or body weight gain. Although DMI for the treatment group was numerically lower than the control group, milk yield, % milk fat, and % milk protein for the treatment group were numerically higher than the control group and resulted in numerically higher 4% FCM and ECM efficiencies for cows received AA supplementation. There was a statistical interaction of treatment and day observed on DMI, OMI, and 4% FCM and ECM production efficiencies indicating that the treatment group maintained a better production efficiency than the control group throughout the experiment. The concentration of ruminal NH<sub>4</sub><sup>+</sup> and the proportion of butyrate were increased, but proportions of acetate and valerate were decreased while the pH, total VFA, proportions of propionate, isobutyrate and isovalerate, and the ratio of acetate to propionate were not affected by supplementing AA.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-0710103-102634 |
| Date | 10 July 2003 |
| Creators | Chung, Yi-Hua |
| Contributors | L Lee Southern, Bruce F Jenny, H. Gale Bateman, II, Cathleen Collett Williams |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-0710103-102634/ |
| Rights | unrestricted, I hereby grant to LSU or its agents the right to archive and to make available my thesis or dissertation in whole or in part in the University Libraries in all forms of media, now or hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation. |
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