Considerable variation in the percentage of Y-chromosome bearing spermatozoa
(%Y-CBS) between ejaculates in the same male has been reported. Collection regime has
been speculated to influence the degree of variation in %Y-CBS in bull ejaculates
(Chandler et al., 1998). Experiments were designed to evaluate the effects of two
collection regimes on %Y-CBS. Conventional PCR combined with gel electrophoresis
and image analysis was employed to determine %Y-CBS in ejaculates from two bulls
collected on 7-day intervals and two bulls on 21-day intervals. Real-time PCR technology
was used to quantify %Y-CBS in the same ejaculates. Boar ejaculates were also analyzed
with both techniques and compared to the percent male piglets in litters resulting from the
assayed ejaculates. Collection day (P<0.0001) significantly affected %Y-CBS as
determined by both PCR methods. Ejaculate nested within bull (P<0.07) was significant in
the conventional PCR study and collection frequency (P<0.0001) in the realtime PCR trial.
Ejaculate nested within boar was highly significant (P<0.0001) for both technologies.
Boar was significant (P<0.002) in the conventional PCR study. Predicted %Y-CBS
determined by real-time PCR was significantly correlated (0.52, P=0.004) to percent male
piglets. Both PCR techniques were effective in quantifying the variation in %Y-CBS in
bull and boar ejaculates. Manipulation of %Y-CBS via collection regime may prove
valuable for altering the secondary sex ratio in animal agriculture.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-1112103-071121 |
| Date | 12 November 2003 |
| Creators | Paul, Julius Braden |
| Contributors | Shulin Li, John E. Chandler, Donald L. Thompson, Richard K. Cooper, Sue G. Bartlett |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-1112103-071121/ |
| Rights | unrestricted, I hereby grant to LSU or its agents the right to archive and to make available my thesis or dissertation in whole or in part in the University Libraries in all forms of media, now or hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation. |
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