Yung Cheuk Man. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 89-113). / Abstracts in English and Chinese. / Abstract (in English) --- p.i / Abstract (in Chinese) --- p.iii / Acknowledgement --- p.v / Table of content --- p.vii / List of figures --- p.xi / Symbols and abbreviations --- p.xiii / Scientific names --- p.xv / Chapter Chapter 1 --- General Introduction --- p.1 / Chapter 1.1 --- Structure of ovarian follicles --- p.2 / Chapter 1.2 --- Steroidogenesis in the ovary --- p.3 / Chapter 1.2.1 --- Two-cell-type model --- p.5 / Chapter 1.2.2 --- Steroidogenic shift --- p.8 / Chapter 1.3 --- Aromatase --- p.8 / Chapter 1.3.1 --- Structure --- p.8 / Chapter 1.3.2 --- Function --- p.9 / Chapter 1.3.3 --- Mechanism of aromatase action --- p.11 / Chapter 1.3.4 --- Expression --- p.13 / Chapter 1.3.5 --- Regulation --- p.14 / Chapter 1.3.5.1 --- Gonadotropins --- p.15 / Chapter 1.3.5.2 --- Insulin-like growth factor-I --- p.17 / Chapter 1.3.5.3 --- Activin --- p.19 / Chapter 1.4 --- Objectives of the present study --- p.23 / Chapter Chapter 2 --- Expression profiles of the ovarian aromatase in the zebrafish --- p.25 / Chapter 2.1 --- Introduction --- p.25 / Chapter 2.2 --- Materials and Methods --- p.27 / Chapter 2.2.1 --- Animals --- p.27 / Chapter 2.2.2 --- Total RNA extraction from intact ovaries and ovarian follicles --- p.27 / Chapter 2.2.3 --- Validation of semi-quantitative RT-PCR assays for aromatase and GAPDH --- p.28 / Chapter 2.2.4 --- Data analysis --- p.29 / Chapter 2.3 --- Results --- p.30 / Chapter 2.3.1 --- Validation of the semi-quantitative RT-PCR assays for aromatase and GAPDH --- p.30 / Chapter 2.3.2 --- Developmental expression profile of aromatase in the whole ovary during sexual maturation --- p.32 / Chapter 2.3.3 --- Stage-dependent expression of aromatase in the ovarian follicles of mature gravid zebrafish --- p.35 / Chapter 2.4 --- Discussion --- p.37 / Chapter Chapter 3 --- Regulation of aromatase expression in vitro --- p.42 / Chapter 3.1 --- Introduction --- p.42 / Chapter 3.2 --- Materials and Methods --- p.45 / Chapter 3.2.1 --- Animals --- p.45 / Chapter 3.2.2 --- Chemicals and hormones --- p.45 / Chapter 3.2.3 --- Preparation of goldfish pituitary extract --- p.45 / Chapter 3.2.4 --- Primary follicle cell culture --- p.46 / Chapter 3.2.5 --- Preparation of freshly isolated mid-vitellogenic follicles --- p.46 / Chapter 3.2.6 --- Preparation of ovarian fragments --- p.47 / Chapter 3.2.7 --- "Total RNA extraction from cultured follicle cells, ovarian follicles and ovarian fragments" --- p.47 / Chapter 3.2.8 --- Validation of semi-quantitative RT-PCR assays --- p.48 / Chapter 3.2.9 --- Data analysis --- p.48 / Chapter 3.3 --- Results --- p.49 / Chapter 3.3.1 --- Validation of the semi-quantitative RT-PCR assays for aromatase and GAPDH --- p.49 / Chapter 3.3.2 --- Expression of aromatase in the zebrafish primary follicle cell culture system --- p.52 / Chapter 3.3.3 --- Gonadotropin regulation of aromatase expression in the zebrafish ovarian fragments and freshly isolated intact follicles --- p.54 / Chapter 3.3.4 --- Effects of db-cAMP and forskolin on aromatase expression in cultured zebrafish follicle cells --- p.59 / Chapter 3.3.5 --- Involvement of protein kinase A (PKA) in the regulation of aromatase expression by db-cAMP in cultured zebrafish follicle cells --- p.64 / Chapter 3.3.6 --- Effects of insulin-like growth factor I (IGF-I) on aromatase expression in zebrafish ovarian fragments --- p.66 / Chapter 3.3.7 --- Effects of activin on aromatase expression in zebrafish ovarian fragments --- p.68 / Chapter 3.4 --- Discussion --- p.71 / Chapter Chapter 4 --- General Discussion --- p.78 / Chapter 4.1 --- Expression profiling of aromatase in the zebrafish ovarian and follicle development --- p.81 / Chapter 4.2 --- Mechanisms for the dynamic expression of aromatase --- p.84 / Chapter 4.3 --- Contribution of the present study --- p.87 / Chapter 4.3 --- Future prospects --- p.88 / References --- p.89
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324432 |
| Date | January 2003 |
| Contributors | Yung, Cheuk Man., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xv, 113 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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