This study was designed to investigate Plantago major leaves, which is widely used
in ethnobotanical uses in Turkey as is the case in all over the world, for their
antioxidant capacities and antimicrobial effect on two gram-positive and two gramnegative
bacteria.
Extracts of P. major leaves prepared in the methanol and fraction samples were
collected in solvents in increasing polarity, from non-polar to polar, to separate
bioactive compound in crude extract. Antioxidant capacities of the crude extracts and
fractions were examined by radical scavenging methods, namely 2,2-azinobis(3-
ethylbenzothiazoline-6-sulfonic acid) ABTS and 1,1-diphenyl-2-picryl-hydrazyl
(DPPH) / as well, total phenolic and total flavonoid contents were determined.
Furthermore, antimicrobial activities of crude extract and fractions were calculated in
terms of minimum inhibitory concentration (MIC) and minimum bactericidal
concentration (MBC) methods together with disc diffusion method.
v
Among the investigated samples, ethyl acetate phase had the highest radical
scavenging capacity with the ABTS value of 2130.4± / 9.845 &mu / mol equivalents of
trolox/ g of ethyl acetate fraction DW, and with EC50 value of 0.011± / 0.001 mg/mL / further, the highest phenolic content was found in ethyl acetate phase with values of
0.452 GAE mg/mg of ethyl acetate fraction DW and 0.434± / 0.0017 mg catechin
equivalent in per mg of ethyl acetate fraction DW.
In general, it was found that the crude extract and aqueous phase of Plantago major
had a weak antimicrobial effect on S. aureus, P.mirabilis, S.pyogenes and E. coli,
along with the highest bacteriostatic and bactericidal upshot on selected bacterial
populations in ethyl acetate fraction.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12613757/index.pdf |
| Date | 01 September 2011 |
| Creators | Kaya, Ozge |
| Contributors | Coruh, Nursen |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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