This thesis examines the challenges of decellularizing porcine lungs. The thesis identifies hydrostatic pressure and the role it plays in perfusate distribution within the lung as a potential roadblock for the decellularization of porcine lungs. The thesis examines methods for mitigating the affect of hydrostatic pressure on the decellularization of porcine lungs. Specifically, the thesis details the design and construction of a vessel that rotates lungs during decellularization. This rotating vessel is evaluated in comparison to two other, non-rotation based, decellularization methods. The rotation device is found to more extensively decellularize the lung than either of the other two options. The thesis concludes by examining what other areas of work could be conducted in this field.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/33422 |
| Date | 22 November 2012 |
| Creators | Frost, Geoffrey |
| Contributors | Waddell, Thomas K. |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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