by Luk Choi Wan. / Thesis submitted in: November 2005. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 125-132). / Abstracts in English and Chinese. / Abstract --- p.iv / 摘要 --- p.vi / Acknowledgements --- p.viii / Tabel of contents --- p.x / List of Figures --- p.xii / List of Tables --- p.xiv / List of Abbreviations --- p.xv / List of Abbreviations --- p.xv / Chapter Chapter One --- Literature review --- p.1 / Chapter 1.1 --- General introduction --- p.1 / Chapter 1.2 --- General aspects of plant lectins --- p.4 / Chapter 1.3 --- Monocot mannose-binding lectins --- p.5 / Chapter 1.3.1 --- General introduction --- p.5 / Chapter 1.3.2 --- Sugar specificity --- p.6 / Chapter 1.3.3 --- Isolation and purification --- p.7 / Chapter 1.3.4 --- Molecular cloning --- p.9 / Chapter 1.3.5 --- Molecular structure and modifications --- p.10 / Chapter 1.3.6 --- Molecular evolution --- p.12 / Chapter 1.3.7 --- Transformation --- p.12 / Chapter 1.3.8 --- Physiological roles --- p.14 / Chapter 1.3.9 --- Application --- p.19 / Chapter 1.4 --- Dendrobium nobile --- p.31 / Chapter 1.4.1 --- Background --- p.31 / Chapter 1.4.2 --- Chemical analysis --- p.32 / Chapter Chapter Two --- Biological activities of a mannose-binding lectin isolated from Dendrobium mobile --- p.33 / Chapter 2.1 --- Introduction --- p.33 / Chapter 2.2 --- Materials and methods --- p.35 / Chapter 2.2.1 --- Mannose-binding lectin from D. nobile --- p.35 / Chapter 2.3 --- Biological activities of mannose-binding lectin from D. nobile --- p.36 / Chapter 2.3.1 --- Hemagglutinating activity --- p.36 / Chapter 2.3.2 --- In vitro anti-proliferative assay --- p.37 / Chapter 2.3.3 --- In vitro antiviral assay --- p.40 / Chapter 2.3.4 --- Statistical Analysis --- p.42 / Chapter 2.4 --- Results --- p.43 / Chapter 2.4.1 --- Physiochemical properties of D. nobile lectins --- p.43 / Chapter 2.4.2 --- Cytotoxicity to cancer cell lines --- p.53 / Chapter 2.4.3 --- Antiviral activity --- p.59 / Chapter 2.5 --- Discussion --- p.60 / Chapter Chapter Three --- Molecular cloning of lectin gene of Dendrobium nobile --- p.65 / Chapter 3.1 --- Introduction --- p.65 / Chapter 3.2 --- Methods --- p.67 / Chapter 3.2.1 --- RNA extraction --- p.67 / Chapter 3.2.2 --- RT-PCR synthesis of D. nobile lectin cDNA --- p.68 / Chapter 3.2.3 --- RACE of D. nobile agglutinin gene --- p.69 / Chapter 3.2.4 --- Generation of DNL full-length lectin gene sequence --- p.70 / Chapter 3.2.5 --- Cloning and sequencing of PCR product --- p.71 / Chapter 3.2.6 --- Data analyses --- p.72 / Chapter 3.2.7 --- Synthesis of single-stranded DIG-labeled DNA probe --- p.74 / Chapter 3.2.8 --- Northern blot analysis --- p.74 / Chapter 3.2.9 --- Genomic DNA extraction --- p.75 / Chapter 3.2.10 --- Southern blot analysis --- p.76 / Chapter 3.2.11 --- Expression of DNL in E. coli --- p.76 / Chapter 3.2.12 --- Western blot analysis --- p.78 / Chapter 3.3 --- Results --- p.80 / Chapter 3.3.1 --- Isolation and characterization of DNL gene --- p.80 / Chapter 3.3.2 --- Sequence analysis of DNL --- p.89 / Chapter 3.2.3 --- Secondary and tertiary structure --- p.96 / Chapter 3.2.4 --- Southern blot analysis --- p.99 / Chapter 3.2.5 --- Northern blot analysis --- p.99 / Chapter 3.2.6 --- Expression of fusion protein in E.coli --- p.102 / Chapter 3.4 --- Discussion --- p.104 / Chapter Chapter Four --- General Discussion --- p.110 / Chapter 4.1 --- General discussion --- p.110 / Chapter 4.2 --- Isolation and Characterization of monocot mannose-binding lectin of D. nobile --- p.111 / Chapter 4.3 --- Molecular cloning of monocot mannose-binding lectin of D. nobile --- p.115 / Chapter 4.4 --- Further investigations --- p.122 / Chapter Chapter Five --- Conclusion --- p.124 / References: --- p.125 / Appendix --- p.133
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_325468 |
| Date | January 2006 |
| Contributors | Luk, Choi Wan., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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