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Authentication by molecular method of dendrobium used in Chinese medicine.

by Lau Tai Wai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 117-127). / Abstracts in English and Chinese. / Table of Content --- p.i / Abbreviations --- p.iv / Abstract --- p.v / List of Figures --- p.ix / List of Tables --- p.xii / Chapter 1. --- Chapter One: Introduction --- p.1 / Chapter 1.1 --- Background on orchids --- p.2 / Chapter 1.2 --- Background on Dendrobium --- p.7 / Chapter 1.3 --- Background and history on Herba Dendrobii --- p.9 / Chapter 1.4 --- Reasons for study of Herba Dendrobii --- p.12 / Chapter 1.4.1 --- Demand --- p.12 / Chapter 1.4.2 --- Adulteration --- p.13 / Chapter 1.4.3 --- CITES --- p.13 / Chapter 1.5 --- Scientific researches on Herba Dendrobii --- p.14 / Chapter 1.5.1 --- Morphological studies --- p.15 / Chapter 1.5.2 --- Anatomical and microscopic studies --- p.16 / Chapter 1.5.3 --- Phytochemistry --- p.20 / Chapter 1.5.3.1 --- Chemicals identified --- p.20 / Chapter 1.5.3.2 --- Chemical authentication of Herba Dendrobii --- p.23 / Chapter 1.5.3.3 --- Effect of treatment on chemical composition --- p.23 / Chapter 1.5.4 --- Phylogenetic study of Dendrobium --- p.25 / Chapter 1.5.4.1 --- Phylogenetic analysis by molecular methods --- p.25 / Chapter 1.5.4.2 --- Phylogenetic analysis by anatomical methods --- p.27 / Chapter 1.5.5 --- Pharmacological effect --- p.29 / Chapter 2. --- Chapter two: Objectives and strategies --- p.30 / Chapter 3. --- Chapter Three: Materials and Methods --- p.33 / Chapter 3.1 --- Source of samples and their treatment --- p.34 / Chapter 3.1.1 --- Fresh materials --- p.34 / Chapter 3.1.2 --- Dry materials --- p.34 / Chapter 3.1.3 --- Outgroup species --- p.35 / Chapter 3.2 --- Experimental protocol --- p.40 / Chapter 3.2.1 --- Rationale of the experiment --- p.40 / Chapter 3.2.2 --- DNA extraction --- p.41 / Chapter 3.2.2.1 --- Cetyltrimethylammonium bromide extraction method --- p.41 / Chapter 3.2.2.1a --- Reagents and buffers --- p.41 / Chapter 3.2.2.1b --- Procedures of CTAB extraction method --- p.42 / Chapter 3.2.2.2 --- Modified DNA isolation protocol for dry samples --- p.43 / Chapter 3.2.2.2a --- Reagents and buffers --- p.43 / Chapter 3.2.2.2b --- Procedures of modified DNA isolation protocol for dry plant samples --- p.44 / Chapter 3.2.3 --- Agarose gel electrophoresis of genomic DNA or PCR products --- p.45 / Chapter 3.2.3a --- Reagents and buffers --- p.45 / Chapter 3.2.3b --- Procedures of agarose gel electrophoresis of genomic DNA or PCR products --- p.45 / Chapter 3.2.4 --- Qualitative and quantitative analysis of DNA --- p.46 / Chapter 3.2.5 --- Amplification of the internal transcribed spacer 2 (ITS 2) region by Polymerase Chain Reaction --- p.47 / Chapter 3.2.5a --- Internal transcribed spacer 2 (ITS 2) region --- p.47 / Chapter 3.2.5b --- Procedures of polymerase chain reaction of ITS 2 region --- p.48 / Chapter 3.2.6 --- Purification of PCR products or cycle sequencing products --- p.48 / Chapter 3.2.6.1 --- Ethanol precipitation --- p.48 / Chapter 3.2.6.2 --- GENECLEAN® protocols --- p.49 / Chapter 3.2.6.3 --- Spin Column Purification --- p.49 / Chapter 3.2.7 --- Cycle Sequencing --- p.50 / Chapter 3.2.8 --- Sample Electrophoresis --- p.51 / Chapter 3.2.8a --- Equipment and reagents --- p.51 / Chapter 3.2.8b --- Procedures of sample electrophoresis --- p.52 / Chapter 3.2.9 --- Sequence analysis --- p.52 / Chapter 4. --- Results --- p.53 / Chapter 4.1 --- Fresh materials --- p.54 / Chapter 4.1.1 --- Genomic DNA --- p.54 / Chapter 4.1.2 --- PCR products --- p.59 / Chapter 4.1.3 --- Sequence alignment --- p.66 / Chapter 4.1.4 --- Comparison of the sequences --- p.94 / Chapter 4.1.5 --- Percentage difference among Dendrobium --- p.96 / Chapter 4.1.6 --- Intra-specific variation of orchid species --- p.96 / Chapter 4.1.7 --- Phylogenetic analysis --- p.99 / Chapter 4.2 --- Dry materials --- p.101 / Chapter 4.2.1 --- Genomic DNA --- p.101 / Chapter 4.2.2 --- PCR products --- p.101 / Chapter 4.2.3 --- Sequencing result --- p.101 / Chapter 5. --- Discussion and Conclusion --- p.107 / Chapter 5.1 --- Reasons for authentication of Herba Dendrobii --- p.108 / Chapter 5.2 --- Fresh materials of Herba Dendrobii --- p.109 / Chapter 5.2.1 --- Authentication --- p.109 / Chapter 5.2.2 --- Phylogenetic analysis --- p.111 / Chapter 5.3 --- Dry materials of Herba Dendrobii --- p.114 / Chapter 5.4 --- Evaluation of the experimental method --- p.115 / Chapter 5.5 --- Conclusion --- p.116 / Chapter 6. --- Reference --- p.117 / Chapter 7. --- Appendix / Appendix 1: Number of species in each medicinal orchid geneus --- p.128 / Appendix 2: Photographs showing 15 of the 17 species of orchids used in this research project --- p.131

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323191
Date January 2000
ContributorsLau, Tai Wai., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xii, 135 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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