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Isolation from soil and characterization of a denitrifying Cytophaga capable of reducing nitrous oxide in the presence of acetylene and sulfide

This study investigated possible reasons for the failure of the acetylene inhibition assay of denitrification in highly reducing environments and resulted in the isolation of a denitrifying Cytophaga from soil enriched by anaerobic incubation with glucose, nitrous oxide (N(,2)O), acetylene (C(,2)H(,2)), and sulfide (S('2-)). Such soil enrichments and pure cultures of the isolated Cytophaga (Is-11) reduced N(,2)O rapidly even in the presence of a normally inhibitory concentration of C(,2)H(,2) (4 kPa), providing S('2-) was present 8.0 umol (g soil)('-1) or 0.4 umol (mL culture)('-1) . The observed reaction appears to be unique to this soil Cytophaga since other organisms tested, using the same or similar experimental conditions, failed to show this response. / The isolate was characterized, after extensive comparative studies with five Cytophaga johnsonae strains, as a pigmented, Gram-negative, non-motile, gliding filamentous bacillus. Although these features established a taxonomic link with the family Cytophagaceae, the inability of Is-11 to digest chitin and DNA base composition of about 42.5 mol% (G+C) make the organism's specific affiliation uncertain.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.72068
Date January 1985
CreatorsAdkins, Anne M.
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Microbiology.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 000230950, proquestno: AAINL24071, Theses scanned by UMI/ProQuest.

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