Female ageing leads to reduced hair density and thinner fibres. The impact of
the ageing dermal environment on the hair follicles (HFs) remains unclear. This
study documents in situ changes in human female scalp skin of women (19-81
years (yrs)), and corresponding primary cultures of dermal fibroblast (DF) and
dermal sheath (DS) cells.
In situ, the papillary/reticular boundary was indistinguishable in young scalp, but
delineated over 40yrs, with reduced rete ridges, changes in collagen
organisation, reduced podoplanin (PDPN) and increased versican (VCAN)
expression. Hyaluronic acid synthase 2 (HAS2) was highly expressed
throughout the scalp.
Matrix Metallopeptidase 1 (MMP1) and Metallopeptidase inhibitor 1 (TIMP1),
cyclin-dependent kinase inhibitor 2A (p16INK4A), 11β-Hydroxysteroid
dehydrogenase type 1 and 2 (11β-HSD1/HSD11B1 and 11β-HSD2/HSD11B2)
mRNA expression increased in aged DFs. In DS cells, HAS2, Vimentin (VIM) ,PDPN, Lysosomal-associated membrane protein 1 (LAMP1), Sequestosome-
1 (P62) and Protease nexin-1 (SERPINE2) increased, while α-smooth muscle
actin (aSMA) decreased. Both cell types exhibited elevated cartilage oligomeric
protein (COMP) mRNA expression.
Proteomics revealed elevated COMP expression in the DF secretome with age,
suggesting a more fibrotic phenotype or DS migration into the dermis. DF and
DS lysate protein expression suggests altered extracellular matrix (ECM)
remodelling due to increased levels of MMP-2 and Protease inhibitor
Plasminogen activator inhibitor-1 (Serpin E1/PAI-1). Cathepsin C/DPPI protein
lysate expression decreased in DFs but increased in DS.
In summary, ageing female scalp shows striking structural and biological
changes in the HF environment that may impact hair growth, due to alterations
in ECM, senescence and autophagy associated biomarkers.
| Identifer | oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/19911 |
| Date | January 2022 |
| Creators | Williams, Rachel |
| Contributors | Thornton, M. Julie, Tobin, Desmond J. |
| Publisher | University of Bradford, Centre for Skin Sciences. Faculty of Life Sciences |
| Source Sets | Bradford Scholars |
| Language | English |
| Detected Language | English |
| Type | Thesis, doctoral, PhD |
| Rights | <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-nd/3.0/88x31.png" /></a><br />The University of Bradford theses are licenced under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/">Creative Commons Licence</a>. |
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