<p>Dynein is a minus-end directed microtubule motor complex that is required for a diverse range of biological processes, from intracellular-cargo transport to cell migration. Dynein is regulated at multiple levels and its functions are dictated by its affinity for microtubules, motor action, associations with specific cargos, and subcellular localization.</p>
<p>During <i>Drosophila</i> spermatogenesis, dynein localizes to the nuclear surface at entry into meiotic prophase where it plays an essential role in mediating nucleus-centrosome coupling. Dynein also localizes to the nuclear surface of early postmeiotic spermatids, where it maintains associations between the nucleus and basal body and controls the position of the nucleus during elongation. I have identified the conserved gene <i>asunder</i> (<i>asun</i>) as a novel regulator of dyneins localization to the nuclear surface during <i>Drosophila</i> spermatogenesis. In addition, I have found that the dynein regulatory factor, <i>Lis-1</i>, also plays an essential role in promoting the localization of dynein to the nuclear surface, and that it may cooperate with <i>asun</i> in this process.</p>
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-09142009-161041 |
| Date | 22 September 2009 |
| Creators | Anderson, Michael Andrew |
| Contributors | Laura Lee, Ethan Lee, David Miller, Karen Hales, Daniela Drummond-Barbosa |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-09142009-161041/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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