This project is concerned with understanding the role of the mRNA-binding protein Nab2 in the process of nuclear mRNA export. In this dissertation I characterize changes that occur to Nab2s structure and localization during cellular response to increased growth temperatures, known as the heat shock response. I show that Nab2 is both phosphorylated by the MAP kinase Slt2, and re-localizes to discrete intranuclear foci during heat shock. Further, I show that these changes are involved in nuclear retention of mRNA during heat shock, and that loss of the Nab2 kinase results in lack of this retention. In summary, this work demonstrates that Nab2, and the MAP kinase Slt2, are involved in nuclear retention of mRNA during heat shock.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-11302010-140606 |
| Date | 04 December 2010 |
| Creators | Carmody, Sean Robert |
| Contributors | Kathleen Gould, Anna Means, Ronald Emeson, Daniel Liebler |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu//available/etd-11302010-140606/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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