The role of Four jointed box 1 (FJX1) in colorectal cancer (CRC) is presented in this dissertation. FJX1 was identified as a candidate gene for regulating tumor formation in CRC as it was inhibited in rectal cancers after one week treatment with celecoxib. FJX1 mRNA and protein are upregulated in human CRC, and high expression of FJX1 is associated with poor patient prognosis. Novel FJX1 antibodies and expression vectors were developed and used to characterize recombinant FJX1 in vitro. In vivo, FJX1 promotes tumor formation by increasing tumor cell proliferation and vascularization. In vitro, conditioned media from FJX1 expressing cells promoted endothelial cell capillary tube formation in a HIF1-α dependent manner. In addition to these experimental observations, microarray expression profiling of CRC cells with manipulated FJX1 expression is also presented. In sum, these results support the conclusion that FJX1 is a novel regulator of tumor progression, due in part, to its effect on tumor vascularization.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-09302013-132645 |
| Date | 09 October 2013 |
| Creators | Al-Greene, Nicole Theresa |
| Contributors | R. Daniel Beauchamp, James Goldenring, Susan Wente, Albert Reynolds |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-09302013-132645/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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