The genome of a eukaryotic cell tightly packed within the nucleus with a high degree of structural organization. Two mechanisms accounting for nuclear structure and the dynamics of subnucler organization in S. cerevisiae are presented within. First, two powerful genetic screens identify requirements for the RSC chromatin-remodeling complex in maintaining nuclear morphology. The major NE-malformations observed in rsc mutants likely result from aberrant transcription and lipid homeostasis. Second, nuclear organization of transcriptional events in response to osmotic stress in S. cerevisiae involves the relocalization of the Hot1 transcription factor to foci that overlap with corresponding target genes. Casein Kinase II negatively regulates Hot1 localization to foci, and also leads to a reduced transcriptional response. These results suggest that the nuclear organization of transcription events impact the stochastic activity of environmentally induced genes. In conclusion, both chromatin organization and transcription events result in dynamic alterations in nuclear structure impacting the output of the genome.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-11262013-142751 |
| Date | 04 December 2013 |
| Creators | Burns, Laura Titus |
| Contributors | Dr. William Tansey, Dr. P. Anthony Weil, Dr. Melanie Ohi, Dr. Todd Graham, Dr. Susan Wente |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-11262013-142751/ |
| Rights | restricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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