Naked family members (Drosophila Naked Cuticle and mammalian Naked1 and Naked2) have been identified as inducible antagonists of canonical Wnt signaling. We previously reported that Naked2, but not Naked1, interacts with the cytoplasmic tail of transforming growth factor Ñ (TGFÑw, thereby coating TGFÑ-containing exocytic vesicles and directing these vesicles to the basolateral corner of polarized epithelial cells. We have designated Naked2 a cargo recognition and targeting (CaRT) protein required for TGFÑ basolateral cell surface delivery. Despite the importance of its role in both Wnt signaling and TGFÑ trafficking, the regulation of Naked2 has not been characterized. Moreover, in vivo roles for Naked2 in these biological processes are largely unknown. To address these deficiencies, I have examined the regulation of Naked2 expression in the context of canonical Wnt signaling and epidermal growth factor receptor (EGFR) signaling, and I have generated a targeted conditional disruption of Naked2 in the mouse.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-03182008-133115 |
| Date | 19 March 2008 |
| Creators | Ding, Wei |
| Contributors | Scott Hiebert, James Goldenring, Ethan Lee, Stephen Hann |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-03182008-133115/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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