The work contained in this document describes the generation of new immunoreagents for Bves study, and our identification of a Bves-interacting protein. After generating new monoclonal antibodies to the Bves protein, I here describe the characterization of Bves expression throughout mouse embryogenesis, clearly demonstrating that Bves is widely expressed in many tissue types throughout gestation. I also describe the isolation of Geft as a directly interacting protein for Bves, and biochemically confirm and characterize the interaction between these two proteins. I then demonstrate a role for Bves in control of cellular motility via modulation of the Rac1/Cdc42 GTPases, likely through the Geft interaction. These findings represent both the first identification of a Bves-interacting protein and the first placement of Bves into a molecular pathway within the cell. The data presented here will undoubtedly serve as a departure point for many future investigations into the Bves protein, and represent seminal findings in the study of Bves function.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-04022007-172202 |
| Date | 11 April 2007 |
| Creators | Smith, Travis Kirk |
| Contributors | Chris Wright, David Bader, Scott Baldwin, Roland Stein, Tsutomu Kume |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-04022007-172202/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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