Dynein, a microtubule motor protein complex, plays critical roles in cell-cycle progression in many systems. The dynein accessory factor <i>LIS1</i>, first identified as a causative factor of the human brain disorder Lissencephaly when lost in one copy, is essential for a majority of the cellular activities of dynein. To gain insight into the in vivo functions of LIS1, we characterized a male-sterile allele of the <i>Drosophila</i> homolog of human <i>LIS1</i>. We found defects in centrosome migration and attachments in <i>Lis-1</i> spermatocytes and spermatids. The localization pattern of LIS-1 protein throughout <i>Drosophila</i> spermatogenesis mirrors that of dynein, and dynein recruitment to the nuclear surface and spindle poles is severely reduced in <i>Lis-1</i> male germ cells. We previously identified <i>asunder</i> (<i>asun</i>) as a novel regulator of dynein localization during <i>Drosophila</i> spermatogenesis. We present a model in which <i>Lis-1</i> and <i>asun</i> cooperate to regulate dynein localization and centrosome positioning during <i>Drosophila</i> spermatogenesis.
Expression of <i>asun</i> is much higher in <i>Drosophila</i> ovaries than in testes. We therefore sought to determine whether ASUN plays roles in oogenesis. We characterized the female germline phenotypes of flies homozygous for a null allele of <i>asun</i> (<i>asun<sup>d93</sup></i>). <i>asun<sup>d93</sup></i> females lay very few eggs, and a majority of these eggs are ventralized, possibly as a result of mislocalization of <i>gurken</i> transcripts, a dynein-regulated step, within <i>asun<sup>d93</sup></i> oocytes. Dynein localization and dynein-mediated processes are disrupted in <i>asun<sup>d93</sup></i> oocytes. Taken together, our data indicate that <i>asun</i> is a critical regulator of dynein during <i>Drosophila</i> gametogenesis.
As <i>asun</i> plays a conserved role in regulating dynein during <i>Drosophila</i> gametogenesis, we sought to identify other proteins that cooperate with <i>asun</i> to perform this function. We utilized a set of publicly available 2nd chromosome deficiency lines to initiate a dominant modifier screen to identify genes that could enhance or suppress the <i>asun</i> male phenotype when lost in one copy. Further testing will be required to identify and characterize the individual genes within these deficiency intervals that cooperate with <i>asun</i> to regulate dynein during <i>Drosophila</i> spermatogenesis.
Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-10292013-141930 |
Date | 08 November 2013 |
Creators | Sitaram, Poojitha |
Contributors | David M. Bader, Matthew J. Tyska, Katherine L. Friedman, David M.Miller III, Laura A. Lee |
Publisher | VANDERBILT |
Source Sets | Vanderbilt University Theses |
Language | English |
Detected Language | English |
Type | text |
Format | application/pdf |
Source | http://etd.library.vanderbilt.edu/available/etd-10292013-141930/ |
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