Master’s thesis DNA markers for bacterial infection diagnosis engages in matters of pathogenic bacteria that cause urinary tract infections and in matters of diagnostic methods for this bacteria with focus on the usage of DNA probes. The aim of this thesis was to find, design and test suitable DNA probes by using quantitative Real-Time PCR. Probes were designed based on specific bacterial sequences of 16S rRNA gene fragment. Afterwards, testing and optimisation of Real-Time PCR was conducted. Successful optimisation was managed for two probes out of twelve – one for S. aureus, second for P. aeruginosa. These results represent the basis for the follow-up experimental work to ensure improvement and reproducibility of the experiments.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:427269 |
| Date | January 2018 |
| Creators | Vilímová, Veronika |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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