by Kwan Yee Ting, Chris. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 44-48). / Abstract also in Chinese. / Acknowledgements --- p.i / Abstract --- p.ii / Table of Contents --- p.v / List of Figures --- p.ix / List of Tables --- p.x / Chapter Part 1. --- Introduction --- p.1 / Chapter 1.1 --- Identification of TCM --- p.1 / Chapter 1.2 --- Chemical Pattern Recognition --- p.2 / Chapter 1.3 --- Discriminant Analysis --- p.3 / Chapter 1.4 --- Epimedium --- p.5 / Chapter 1.5 --- High Performance Liquid Chromatography --- p.6 / Chapter 1.6 --- Objectives of this work --- p.8 / Chapter Part 2. --- Chemical Analysis --- p.9 / Chapter 2.1 --- Sources of Epimedium samples --- p.9 / Chapter 2.2 --- Extraction --- p.9 / Chapter 2.2.1 --- Sample Pre-treatment --- p.9 / Chapter 2.2.2 --- Extraction Procedure --- p.9 / Chapter 2.2.3 --- Extraction Recovery --- p.11 / Chapter 2.3 --- Instrumental Analysis --- p.11 / Chapter 2.3.1 --- Chromatographic Operating Conditions --- p.12 / Chapter 2.3.2 --- Preparation of Calibration Graph --- p.12 / Chapter 2.3.3 --- Sample injection --- p.13 / Chapter 2.4 --- Results and Discussion --- p.13 / Chapter 2.4.1 --- Linearity of the Calibration Graph --- p.13 / Chapter 2.4.2 --- Development of Analysis Procedure --- p.15 / Chapter 2.4.2.1 --- Sample Pre-treatment --- p.15 / Chapter 2.4.2.2 --- Extractant --- p.15 / Chapter 2.4.2.3 --- Purification of Extract --- p.15 / Chapter 2.4.2.4 --- Extraction Time --- p.17 / Chapter 2.4.2.5 --- Solvent Gradient --- p.18 / Chapter 2.4.2.6 --- Detection --- p.19 / Chapter 2.4.3 --- Quantitative Analysis --- p.19 / Chapter 2.4.3.1 --- Extraction Recovery --- p.19 / Chapter 2.4.3.2 --- Icariin Content --- p.20 / Chapter 2.5 --- Conclusions --- p.22 / Chapter Part 3. --- Chemical Pattern Recognition --- p.24 / Chapter 3.1 --- Materials and Methods --- p.24 / Chapter 3.1.1 --- Chromatographic Results --- p.24 / Chapter 3.1.2 --- Patterns of Epimedium Samples --- p.24 / Chapter 3.1.3 --- Computer Program --- p.25 / Chapter 3.1.4 --- Variable Extraction --- p.25 / Chapter 3.1.4.1 --- Variable Extraction Parameters --- p.25 / Chapter 3.1.4.2 --- Variable Extraction Methods --- p.26 / Chapter 3.1.4.3 --- Transformation of Variables --- p.27 / Chapter 3.1.5 --- Variable Selection --- p.27 / Chapter 3.1.6 --- Predictive Power of the Recognition Model --- p.28 / Chapter 3.2 --- Results --- p.28 / Chapter 3.2.1 --- Accuracy of the Recognition Models --- p.28 / Chapter 3.2.2 --- Classification Functions --- p.29 / Chapter 3.2.3 --- Casewise Results of Recognition Model IV --- p.31 / Chapter 3.2.4 --- Plotting of the Best Two Canonical Discriminant Functions --- p.33 / Chapter 3.3 --- Discussion --- p.33 / Chapter 3.3.1 --- Meaning of Extracted Variables --- p.33 / Chapter 3.3.2 --- Limitations of Variable Extraction Methods --- p.34 / Chapter 3.3.3 --- Importance of the Variable Extraction Methods --- p.34 / Chapter 3.3.4 --- "Reasons for the Poor Performance in Recognition Models I, II and III" --- p.35 / Chapter 3.3.5 --- Selected Variables in Model IV --- p.35 / Chapter 3.3.6 --- Misclassified Samples --- p.36 / Chapter 3.3.7 --- Quality Assessment --- p.38 / Chapter 3.3.8 --- Comparison with Another Chemical Pattern Recognition Method for the Identification of Epimedium --- p.39 / Chapter 3.3.9 --- Potential Usage of the Pattern Recognition Method --- p.42 / Chapter 3.3.10 --- Advantage of the Pattern Recognition Method --- p.42 / Chapter 3.3.11 --- Disadvantage of Discriminant Analysis --- p.42 / Chapter 3.4 --- Conclusions --- p.43 / References --- p.44 / Appendix I Epimedium Species in China --- p.49 / Appendix II --- p.50 / Chapter II.1 --- Chromatograms of Samples of Epimedium sagittatum --- p.50 / Chapter II.2 --- Chromatograms of Samples of Epimedium pubescens --- p.57 / Chapter II.3 --- Chromatograms of Samples of Epimedium koreanum --- p.61 / Chapter II.4 --- Chromatograms of Samples of Epimedium leptorrhizum --- p.67 / Chapter II.5 --- Chromatograms of Samples of Epimedium wnshanese --- p.69 / Chapter II.6 --- Chromatograms of Samples of Epimedium brevicornum --- p.72 / Appendix III Log-transformed Values of Variables --- p.75
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322378 |
| Date | January 1998 |
| Contributors | Kwan, Yee Ting Chris., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, x, 83 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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