Stem rust and Yellow rust diseases are the two major wheat fungal diseases causing
considerable yield losses in Turkey and all around the world. There are studies which
are carried out to identify and utilize resistance sources in order to obtain resistant
lines of wheat. However, virulent pathotypes are continuously being important
threats to wheat production and yield. For that reason, new approaches for rapid
identification are needed.
The aim of this study was to investigate and to understand the structural and
functional differences between the resistant and sensitive durum wheat cultivars to
the plant fungal diseases of stem and yellow (stripe) rusts. To aim this, forty durum
wheat recombinant inbred lines (RILs), which were previously determined to be
resistant or sensitive to stem and yellow rust diseases, were investigated by the noninvasive
Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR)
Spectroscopy. Also, classification of the resistant and sensitive lines depending on
the structural and functional differences has been attempted. The FTIR spectra for stem rust disease showed that, resistant durum wheat lines had
a significant increase in the population of unsaturation in acyl chains of lipid
molecules, an increase in lipid and in total protein content and also an increase in
carboxylic acids and alcohols. For yellow rust disease, resistant lines had a
significant increase in hydrogen bonding and they had also a more ordered
membrane structure.
In Principal Component Analysis for stem rust disease, according to 3700-650 cm-1
region, amide III band (1213-1273 cm-1 region) and C-H stretching region (3020-
2800 cm-1), the resistant and sensitive groups were separated successfully. For
yellow rust disease, according to 3700-650 cm-1 region, Amide A and Amide III
bands, the resistant and sensitive lines were grouped distinctly.
FTIR spectroscopy provides a useful approach to determine the differences in
molecular structure of durum wheat RILs regarding resistance of lines to fungal
diseases. However, further research is still needed to ensure if the structural and
functional differences in biomolecules of the samples could be used as molecular
markers for discrimination of rust resistant materials from rust sensitive ones.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12613677/index.pdf |
| Date | 01 September 2011 |
| Creators | Kansu, Cigdem |
| Contributors | Kaya, Zeki |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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