Anthracnose, caused by <i>Colletotrichum truncatum</i>, is a major disease of lentil (<i>Lens culinaris</i> Medik.) on the Canadian prairies. Resistance to the more virulent race Ct0 of the pathogen is extremely rare within the <i>L. culinaris</i> gene pool thus resistance is being introgressed from <i>Lens ervoides</i>. The overall hypothesis of this project was that resistance derived from <i>Lens ervoides</i> accession L-01-827A would provide uniquely low levels of resistance to <i>Colletotrichum truncatum</i>. Individual studies were performed to: i) test field-resistance of interspecific lines; ii) develop a cutting-method to generate replicated phenotypic data on single plants; iii) study the deterioration of resistance between the juvenile phase (JP) and adult phases (AP) in the line CDC Redberry; and iv) examine genetic control of resistance in a susceptible and partially resistant background. Field evaluations of <i>L. culinaris x L. ervoides</i> lines indicated resistance genes from the wild species will provide unprecedented levels of disease control and heritability of the trait was estimated to be moderate. Single plant phenotyping of resistance is unreliable, thus the feasibility of using clonal propagation of individual plants to generate replicated ratings was evaluated. Results showed consistency in segregation ratios between cutting and seedling-derived plants of the same population, thus the method was utilized for testing of genetic control of segregating populations. CDC Redberry showed varying disease levels depending on the age of the plants with resistance acquisition in the JP that decreased as the plants proceeded through the AP. The F<sub>1</sub>, F<sub>2</sub> and F<sub>2:3</sub> generations of two introgression populations were tested for resistance. Resistance from <i>L. ervoides</i> to both races of <i>C. truncatum</i> appeared to be due to the same gene(s) or from the same linkage block in both populations. Models for genetic control were consistent between F<sub>2</sub> and F<sub>2:3</sub> generations, however were different between the populations depending on whether the interspecific line was crossed into a susceptible or partially resistant <i>L. culinaris</i> background. However duplicate recessive epistasis seemed to control susceptibility in the susceptible background and when JP resistance was not a factor. There were significant differences between the JP and AP on more than a third of the F<sub>2</sub>s tested supporting different resistance gene action based on growth phase. Resistance in the JP seemed to be due to dominant and recessive epistasis. It was postulated that the the populations may have had segregation distortion commonly found in interspecific populations, thus the previously described genotypes for cotyledon colour and albino plants were used to test the hypothesis of segregation distortion. Segregation of these traits were found to be similarly distorted to previously reported interspecific <i>L. culinaris x L. ervoides</i> populations supporting the hypothesis that continued segregation distortion was found in the introgression populations. Overall, it was found that resistance derived from <i>L. ervoides</i> accession L-01-827A is a highly effective source for the lentil breeding program, however the result suggest more than one backcross to <i>L. culinaris</i> be necessary to properly integrate the resistance genes to eventually obtain fully fertile, adapted lentil cultivars.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:SSU.etd-09202010-125323 |
| Date | 20 September 2010 |
| Creators | Vail, Sally Lynne |
| Contributors | Vandenberg, Albert, Coulman, Bruce, Banniza, Sabine, McCartney, Curt, Pozniak, Curtis, Wei, Yangdou, Michaels, Tom |
| Publisher | University of Saskatchewan |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://library.usask.ca/theses/available/etd-09202010-125323/ |
| Rights | restricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Saskatchewan or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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