The nematode Caenorhabditis elegans is a unique resource for the study of axon guidance and cell migration. Genetic data collected from C. elegans research is integral to advancing our knowledge of genetic pathways that control axon and cell guidance, such as the UNC-6/netrin pathway, within a developing organism. In this thesis, I describe the characterization of ev821, an allele isolated from a screen for novel guidance mechanisms or components of the UNC-6 pathway. Furthermore, I show that ev821 is a maternal-effect and temperature-sensitive mutation with defects in phase 2 of distal tip cell migration in the C. elegans hermaphrodite, as well as ray-1 displacement and a blister phenotype in the C. elegans male. My research on ev821 could broaden our understanding of the mechanisms driving UNC-6 guidance, and has the potential to identify unknown factors that act within or in parallel to the UNC-6 signaling pathway.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OTU.1807/43325 |
| Date | 10 December 2013 |
| Creators | Veyhl, Joseph |
| Contributors | Culotti, Joseph |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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