Bicaudal-C, a Drosophila gene, is required for centripetal follicle cell migration in the egg chamber and anterior patterning of the embryo; its exact functions are unknown. BICAUDAL-C carries five KH domains required for in vitroRNA-binding and for in vivo activity. Here, I show that Bicaudal-C functions, through RNA-binding, to regulate oskar expression during mid-oogenesis. Females carrying mutations in Bicaudal-C in the regions encoding its KH domains display premature, ectopic translation of OSKAR at the anterior of their stages 8--10 oocytes; this likely accounts for the disruption of anterior patterning in embryos of Bicaudal-C/+ females. Also here, I propose that oskar and gurken share common regulators, including Bicaudal-C. This is based on the discovery of dorsalized progeny of Bicaudal-C/+ females, and on the dominant enhancement of the Bicaudal-C/+ phenotype by specific gurken and cornichon alleles. Finally, I describe a yeast two-hybrid screen used to investigate BICAUDAL-C-protein interaction.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.20851 |
| Date | January 1998 |
| Creators | Rother, Katherine L. |
| Contributors | Lasko, Paul F. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001642371, proquestno: MQ44261, Theses scanned by UMI/ProQuest. |
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