Yim Wan Sze. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 95-109). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Abstract --- p.ii / 槪論 --- p.iv / Table of contents --- p.v / List of abbreviations --- p.xii / List of Figures --- p.xv / List of Tables --- p.xviii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Oxidation stress --- p.1 / Chapter 1.1.1 --- Free radicals --- p.2 / Chapter 1.1.1.1 --- Hydrogen peroxide --- p.3 / Chapter 1.1.1.2 --- Menadione --- p.4 / Chapter 1.1.2 --- Diseases related to oxidative stress --- p.5 / Chapter 1.1.3 --- Liver Injury --- p.5 / Chapter 1.1.4 --- Antioxidants --- p.7 / Chapter 1.1.4.1 --- Importance of antioxidant --- p.7 / Chapter 1.1.4.2 --- Examples of antioxidant --- p.7 / Chapter 1.2 --- Traditional Chinese Medicinal (TCM) formula Wu-zi-yan-zong-wan (WZ) --- p.8 / Chapter 1.2.1 --- The WZ medicinal formula --- p.8 / Chapter 1.2.2 --- Pharmacological actions of WZ --- p.9 / Chapter 1.2.3 --- Pharmacological actions of individual herbs --- p.10 / Chapter 1.2.3.1 --- Fructus Lycii --- p.10 / Chapter 1.2.3.2 --- Semen Cuscuta --- p.11 / Chapter 1.2.3.3 --- Fructus Rubi --- p.12 / Chapter 1.2.3.4 --- Semen Plantaginis --- p.12 / Chapter 1.2.3.5 --- Fructus Schisandrae --- p.13 / Chapter 1.3 --- The relationship between the liver and kidney --- p.14 / Chapter 1.4 --- Objectives of study --- p.15 / Chapter Chapter 2 --- Preparation of Aqueous Extraction of Wu-zi-yan-zong-wan --- p.17 / Chapter 2.1 --- Introduction --- p.17 / Chapter 2.2 --- Materials and Methods --- p.18 / Chapter 2.2.1 --- Preparation of Wu-zi-yan-zong-wan (WZ) --- p.18 / Chapter 2.2.1.1 --- WZ extracts from raw materials (WZ-e) --- p.18 / Chapter 2.2.1.2 --- WZ extracts from commercial available ready-to-use powders (WZ-p) --- p.20 / Chapter 2.3 --- Results --- p.22 / Chapter 2.3.1 --- Extraction Yield for WZ-e --- p.22 / Chapter Chapter 3 --- In vitro Total Antioxidant Capacity of Aqueous Extracts of Wu-zi-yan-zong-wan and its Components --- p.24 / Chapter 3.1 --- Introduction --- p.24 / Chapter 3.1.1 --- Total Antioxidants: Trolox Equavalent Antioxidant Capacity (TEAC) --- p.24 / Chapter 3.1.2 --- Objectives --- p.25 / Chapter 3.2 --- Materials and methods --- p.26 / Chapter 3.2.1 --- Materials --- p.26 / Chapter 3.2.1.1 --- Reagents --- p.26 / Chapter 3.2.1.2 --- Instruments --- p.26 / Chapter 3.2.2 --- Methods --- p.26 / Chapter 3.2.2.1 --- Total Antioxidnats: Trolox Equavalent Antioxidnat Capacity (TEAC) --- p.26 / Chapter 3.2.3 --- Statistical analysis --- p.27 / Chapter 3.3 --- Results --- p.28 / Chapter 3.3.1 --- Antioxidnat Capacity of Trolox --- p.28 / Chapter 3.3.2 --- Antioxidant capacity of WZ-e formula --- p.28 / Chapter 3.3.3 --- Antioxidant capacity of WZ-p formula --- p.28 / Chapter 3.3.4 --- The total antioxidant capacity of WZ-e and its simplified formulae --- p.32 / Chapter 3.3.5 --- The total antioxidant capacity of WZ-p and its simplified formulae --- p.32 / Chapter 3.3.6 --- Synergetic effect of WZ-e and its simplified formulae --- p.34 / Chapter 3.3.7 --- Orthogonal analysis of WZ-e and its simplified formulae on TEAC assay --- p.40 / Chapter 3.4 --- Discussion --- p.42 / Chapter Chapter 4 --- Antioxidant Activity of Aqueous Extracts of Simplified Formulae of Wu-zi-yan-zong-wan in vitro --- p.44 / Chapter 4.1 --- Introduction --- p.44 / Chapter 4.1.1 --- In vitro antioxidant --- p.44 / Chapter 4.1.2 --- Antioxidant effect of Catechins --- p.44 / Chapter 4.1.3 --- MTT assay --- p.45 / Chapter 4.1.4 --- Objectives --- p.46 / Chapter 4.2 --- Materials and methods --- p.47 / Chapter 4.2.1 --- Materials --- p.47 / Chapter 4.2.1.1 --- Cell Culture --- p.47 / Chapter 4.2.1.2 --- Reagents --- p.47 / Chapter 4.2.2 --- Methods --- p.47 / Chapter 4.2.2.1 --- Cell Culture --- p.47 / Chapter 4.2.2.2 --- MTT Cytotoxicity Assay --- p.48 / Chapter 4.2.3 --- Statistical analysis --- p.48 / Chapter 4.3 --- Results --- p.49 / Chapter 4.3.1 --- The effect of WZ-e formula on HepG2 cells --- p.49 / Chapter 4.3.2 --- The effect of hydrogen peroxide on HepG2 cells --- p.49 / Chapter 4.3.3 --- The effect of menadione on HepG2 cells --- p.52 / Chapter 4.3.4 --- The effect of catechin on HepG2 cells --- p.52 / Chapter 4.3.5 --- The effect of WZ-e and its simplified formulae against hydrogen peroxide-induced cytotoxicty on HepG2 cells --- p.55 / Chapter 4.3.6 --- The effect of WZ-e and its simplified formulae against menadione-induced cytotoxicity on HepG2 cells --- p.60 / Chapter 4.3.7 --- The effect of WZ-p on HepG2 cells --- p.65 / Chapter 4.3.8 --- The effect of WZ-p and its simplified formulae against hydrogen peroxide-induced cytotoxicity on HepG2 cells --- p.67 / Chapter 4.3.9 --- The effect of WZ-p and its simplified formulae against menadione-induced cytotoxicity on HepG2 cells --- p.72 / Chapter 4.4 --- Discussion --- p.77 / Chapter Chapter 5 --- Effect of Aqueous Extract of Wu-zi-yan-zong-wan on Menadione-induced Oxidative Damage in Mouse Liver --- p.79 / Chapter 5.1 --- Introduction --- p.79 / Chapter 5.2 --- Materials and methods --- p.81 / Chapter 5.2.1 --- Materials --- p.81 / Chapter 5.2.1.1 --- Animals --- p.81 / Chapter 5.2.1.2 --- Reagents --- p.81 / Chapter 5.2.1.3 --- Apparatus --- p.81 / Chapter 5.2.2 --- Methods --- p.82 / Chapter 5.2.2.1 --- Animal treatments --- p.82 / Chapter 5.2.2.2 --- Collection of samples --- p.82 / Chapter 5.2.2.3 --- Marker enzyme measurement (ALT) --- p.83 / Chapter 5.2.3 --- Statistical analysis --- p.83 / Chapter 5.3 --- Results --- p.84 / Chapter 5.3.1 --- Dose-dependent effect of WZ-e on menadione hepatotoxicity --- p.84 / Chapter 5.3.2 --- Dose-dependent effect of WZ-e on menadione hepatotoxicity as illustrated by histopathological observations --- p.86 / Chapter 5.3.3 --- Analyzing he effect of WZ-e and its simplified formulae on menadione-induced hepatotoxicity by Orthogonal Analysis89 --- p.89 / Chapter 5.4 --- Discussion --- p.91 / Conclusion --- p.93 / References --- p.95
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324768 |
| Date | January 2004 |
| Contributors | Yim, Wan Sze., Chinese University of Hong Kong Graduate School. Division of Chinese Medicine. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xviii, 109 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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