This study examined the effects of a microbubble emulsion (ME) combined with sonic or ultrasonic agitation, on irrigation dynamics and reduction of biofilm bacteria. High-speed imaging was used to characterize the bubble dynamics generated in ME by sonic or ultrasonic agitation within polymer teeth. 5.25% NaOCl or ME was sonically or ultrasonically agitated in canals of extracted teeth with 7-day-old Enterococcus faecalis biofilms. Dentinal shavings were sampled and colony-forming units (CFU) enumerated. Mean log CFU/ml values were analyzed with ANOVA and post-hoc tests. Strongly oscillating and vaporizing bubbles were generated within ME during ultrasonic, but not sonic agitation. Post-treatment biofilm CFU were significantly lower in the ultrasonic agitation (P=0.000) of ME group than in the ultrasonic (P=0.009) and sonic agitation (P=0.006) of NaOCl groups. The synergistic effect of ME combined with ultrasonic agitation enhanced bubble dynamics and reduced E. faecalis biofilm bacteria beyond the level achieved by agitation of NaOCl.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OTU.1807/33233 |
| Date | 20 November 2012 |
| Creators | Halford, Andrew |
| Contributors | Kishen, Anil |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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