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Previous issue date: 2013-11-29 / Studies on dental caries demonstrate inflammation on dental pulp tissues following infection. Cytokines are able to activate those cells linked to inflammatory and immune response thus neutralizing potentially aggressive agents and allowing tissue repair. Some of those cytokines, denominated chemokines, could contribute to the establishment of an immunotherapy able to control those cytokines involved with the triggering of dental pulp inflammation. Research on chronic inflammation has provided some relevant insights. However, the role of certain chemokines on acute inflammation is yet not conclusive. This study attempts to unveil the role of the chemokines CCL5/RANTES, CCR5 and RANKL on initial and acute pulpal inflammation. Methods: 18 Wistar rats were subjected to anesthesia. Access was performed on their first lower molars until pulp exposure. They were divided into three experimental groups considering the solution administered: Saline, lipopolysaccharide (LPS) and lipoteichoic acid (LTA) (n=6 each group). Higid second lower molars were used as controls. The cavities were sealed with amalgam and euthanasia occurred after 48 h and the jaws were dissected for histologic evaluation. CCL5/RANTES is expressed in all groups, unlike CCR5, that was not expressed. RANKL appears only in inflammatory cells, including control group. Only RANKL could be expressed during initial pulpal inflammation, being able to detect inflammatory cell activity. / Estudos de c?rie demonstraram aspectos inflamat?rios nos tecidos pulpares decorrentes de infec??o estabelecida. Citocinas seriam capazes de ativar c?lulas ligadas ? resposta inflamat?ria e imune no sentido de neutralizar potenciais agentes invasores e permitir o reparo dos tecidos pulpares. Algumas destas, denominadas quimiocinas, poderiam contribuir no estabelecimento de uma imunoterapia capaz de bloquear aquelas citocinas respons?veis pelo processo inflamat?rio. Na inflama??o cr?nica estas pesquisas j? possuem resultados satisfat?rios. Na inflama??o aguda, os estudos ainda est?o inconclusivos. Este estudo teve como objetivo testar as quimiocinas CCL5/RANTES, CCR5 e RANKL na inflama??o pulpar aguda. M?todos: 18 Ratos Wistar foram anestesiados. Foram feitas cavidades at? a exposi??o pulpar nos primeiros molares inferiores. Eles foram divididos em tr?s grupos onde se administrou solu??o salina est?ril, lipopolissacar?deo (LPS) e ?cido lipoteic?ico (LTA) (n= 6 por grupo). Os segundos molares inferiores h?gidos foram utilizados como controle. As cavidades foram seladas com am?lgama e a eutan?sia foi realizada ap?s 48h. As mand?bulas foram dissecadas para exame histol?gico e da imunoexpress?o das quimiocinas em estudo. Obteve-se como resultados: CCL5/RANTES ? expressa em todos os grupos, ao contr?rio do CCR5, que n?o foi expressa. RANKL aparece apenas em c?lulas inflamat?rias. Somente a express?o de RANKL foi capaz de demonstrar atividade celular na inflama??o pulpar inicial.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1253 |
| Date | 29 November 2013 |
| Creators | Fredrich, Andr?a Longoni |
| Contributors | Figueiredo, Jos? Antonio Poli de |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 600, 4673435736271820140 |
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