Microbial resource mining of electroactive microorganism (EAM) is currently methodically
hampered due to unavailable electrochemical screening tools. Here, we introduce an
electrochemical microwell plate (ec-MP) composed of a 96 electrochemical deepwell plate
and a recently developed 96-channel multipotentiostat. Using the ec-MP we investigated
the electrochemical and metabolic properties of the EAM models Shewanella oneidensis
and Geobacter sulfurreducens with acetate and lactate as electron donor combined with
an individual genetic analysis of each well. Electrochemical cultivation of pure cultures
achieved maximumcurrent densities (jmax) and coulombic efficiencies (CE) that were well in
line with literature data. The co-cultivation of S. oneidensis and G. sulfurreducens led to an
increased current density of jmax of 88.57 ± 14.04 μA cm−2 (lactate) and jmax of 99.36 ±
19.12 μA cm−2 (lactate and acetate). Further, a decreased time period of reaching jmax and
biphasic current production was revealed and the microbial electrochemical performance
could be linked to the shift in the relative abundance.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84500 |
| Date | 03 April 2023 |
| Creators | Kuchenbuch, Anne, Frank, Ronny, Ramos, José Vazquez, Jahnke, Heinz-Georg, Harnisch, Falk |
| Publisher | Frontiers Research Foundation |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2296-4185, 821734 |
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