This research attempted to develop “label-free” biosensor for the detection of
cardiovascular risk biomarker, C-reactive protein (CRP). An electrospun Polystyrene (PS)/
Polyaniline (PANI) nanofiber was used as the sensing element of the biosensor and coupled with
electrical measurement system. High aspect ratio and porosity of nanoscale fiber provides size
matched confinement for tiny biomolecule immobilization, which leads to rapid transduction and
enhancement in signal strength. Nanofiber mat was integrated with a gold microelectrode
interdigitated chip and microfluidic chamber to create a lab-on-chip platform.
Results demonstrated that nanotextured fiber with appropriate chemical and electrical
functionality can be used as sensing element of an electrochemical biosensor for detection of
biomolecules at very low concentration. CRP was successfully detected at 100 fg/ml
concentration from human serum suggesting the potential of this lab-on-chip platform for early
detection of any cardiovascular risk. A large dynamic linear range of 100 fg/ml-1 μg/ml was
achieved in human serum for detection of CRP. Less than 10% cross-reactivity with human
albumin suggests the selective detection capability of the proposed biosensor. / Thesis (M.S.)--Wichita State University, College of Engineering, Dept. of Mechanical Engineering.
| Identifer | oai:union.ndltd.org:WICHITA/oai:soar.wichita.edu:10057/5052 |
| Date | 07 1900 |
| Creators | Anwar, Md. Rajib |
| Contributors | Asmatulu, Ramazan |
| Publisher | Wichita State University |
| Source Sets | Wichita State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis |
| Format | xiv, 71 p. |
| Rights | © Copyright 2011 by Md. Rajib Anwar. All rights reserved |
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