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Previous issue date: 2013-03-05 / The purpose of this thesis was to evaluate, in vitro, antimicrobial activity and substantivity of chlorhexidine formulations which are used during root canal therapy. Eighty five bovine teeth and forty five human teeth were extracted for antimicrobial and substantivity experiments respectively. The eighty five bovine teeth were inoculated with Enterococcus faecalis in order to provide biofilm formation. The teeth were divided into ten groups according to disinfectant penetration, intracanal dressing and medication placement site. Microbiological test (UFCs counting) and scanning electronic microscopy (SEM) were performed to evaluate and illustrate the efficacy of proposed treatments respectively. The forty five human teeth were divided into three groups according to chemical auxiliary substance used during root canal therapy. The root canals were prepared up to #45 file. Longitudinal grooves were made in the root surface, providing two halves of each root and resulting in thirty samples per group. The samples of each group were randomly divided into three subgroups and substantivity was evaluated after 24 hours, 30 days and 90 days of incubation. The amount of CHX (in μM) was measured through reverse-phase high-performance liquid chromatography. Statistical analysis was performed by analysis of variance and the Tukey test for post hoc comparisons (α = 0.05) in both experiments. According to limitation of experiments from this thesis, it was possible to conclude that chlorhexidine formulations, liquid and gel, can be considered effectives as intracanal dressing against Enterococcus faecalis, when associated to previous disinfectant penetration with 2% sodium hypochlorite. Furthermore, it was possible to conclude that chlorhexidine formulations, liquid and gel, remained into the root canal up to 90 days after chemo-mechanical preparation. / O prop?sito da presente Tese foi analisar, in vitro, a a??o antimicrobiana e a substantividade de diferentes formula??es de clorexidina comumente utilizadas durante o tratamento endod?ntico. Para o presente estudo foram utilizados 85 dentes bovinos e 45 dentes humanos extra?dos para o experimento de a??o antimicrobiana e substantividade respectivamente. Os 85 dentes bovinos foram inoculados com Enterococcus faecalis, permanecendo em cultura por 30 dias para a forma??o do biofilme. Os dentes foram divididos em dez grupos de acordo com a presen?a de penetra??o desinfetante, medica??o intracanal utilizada e o local de coloca??o desta medica??o. . Teste microbiol?gico (contagem de UFCs) e microscopia eletr?nica de varredura (MEV) foram realizados para avaliar e ilustrar respectivamente a efic?cia dos tratamentos propostos. Os 45 dentes humanos foram divididos em tr?s grupos de acordo com a subst?ncia qu?mica auxiliar utilizada no preparo do canal radicular. Os canais radiculares foram preparados apicalmente at? o instrumento #45. Sulcos longitudinais foram confeccionados na superf?cie das ra?zes, proporcionando duas metades de cada ra?z e resultando em 30 amostras por grupo. As amostras de cada grupo foram aleatoriamente divididas em tr?s subgrupos e a substantividade foi avaliada ap?s 24 horas, 30 dias e 90 dias de incuba??o. A quantidade de clorexidina (em μM) foi mensurada atrav?s de cromatografia de fase-reversa de alta performance. An?lise estat?stica foi realizada atrav?s de ANOVA, seguido pelo post-hoc de Tukey (α = 0.05) em ambos experimentos. Diante da limita??o dos estudos da presente tese, foi poss?vel concluir que as formula??es de clorexidina, l?quida e gel, s?o efetivas medica??es intracanais no que diz respeito ao combate ao Enterococcus faecalis, quando associadas ? pr?via penetra??o desinfetante com hipoclorito de s?dio 2%. Al?m disso, foi poss?vel concluir que as formula??es de clorexidina, l?quida e gel, permanecem retidas no interior do canal radicular por at? 90 dias ap?s a realiza??o do preparo qu?mico-mec?nico com estas subst?ncias.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1221 |
| Date | 05 March 2013 |
| Creators | Souza, Matheus Albino |
| Contributors | Figueiredo, Jos? Antonio Poli de |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 600, 4673435736271820140 |
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