Cheung Ka Wai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 83-93). / Abstracts in English and Chinese. / Acknowledgments --- p.i / Publications --- p.ii / Awards --- p.iii / List of abbreviations --- p.iv / List of figures --- p.vi / List of tables --- p.vii / Abstract in English --- p.viii / Abstract in Chinese --- p.ix / Table of Contents / Chapter Chapter 1 --- Introduction --- p.1 / Chapter Chapter 2 --- literature Review / Chapter 2.1 --- Anatomy and Physiology of Endometrium --- p.2 / Chapter 2.2 --- Endometrial cancer --- p.5 / Chapter 2.2.1 --- Epidemiology --- p.6 / Chapter 2.2.2 --- Etiologies and Risk Factors --- p.7 / Chapter 2.3 --- Pathology --- p.11 / Chapter 2.3.1 --- Grading of endometrial cancer --- p.14 / Chapter 2.3.2 --- Staging of endometrial cancer --- p.14 / Chapter 2.4 --- Prevention and Treatment --- p.16 / Chapter 2.5 --- Molecular alterations in endometrial cancer --- p.17 / Chapter 2.5.1 --- Genetic alterations in endometrial cancer --- p.18 / Chapter 2.5.1.1 --- Oncogene activation --- p.19 / Chapter 2.5.1.2 --- Tumor suppressor gene inactivation --- p.20 / Chapter 2.5.1.2.1 --- Mutation and loss of heterozygosity of tumor suppressor genes in endometrial cancer --- p.22 / Chapter 2.5.2 --- Epigenetic alterations --- p.27 / Chapter 2.5.2.1 --- CpG islands methylation --- p.29 / Chapter 2.5.2.2 --- de novo methylation --- p.29 / Chapter 2.5.2.3 --- Detection of gene promoter hypermethylation --- p.31 / Chapter 2.5.2.4 --- Epigenetic alteration in endometrial cancer --- p.31 / Chapter 2.5.2.5 --- Promoter hypermethylation of tumor suppressor genes in other cancers --- p.39 / Chapter 2.5.3 --- Microsatellite instability --- p.42 / Chapter Chapter 3 --- The objectives of study --- p.45 / Chapter Chapter 4 --- Materials and Methods --- p.46 / Chapter 4.1 --- Samples --- p.46 / Chapter 4.1.1 --- Formalin fixed paraffin embedded tissues --- p.46 / Chapter 4.1.2 --- Cell lines --- p.46 / Chapter 4.2 --- Histological grading and staging of samples --- p.47 / Chapter 4.3 --- Microdissection on tissue sections --- p.47 / Chapter 4.4 --- Extraction of nucleic acid / Chapter 4.4.1 --- Extraction of DNA from paraffin-embedded tissues --- p.48 / Chapter 4.4.2 --- Extraction of DNA from cell lines --- p.49 / Chapter 4.5 --- DNA methylation analysis --- p.49 / Chapter 4.5.1 --- Overview of Methylation-Specific PCR (MSP) --- p.49 / Chapter 4.5.2 --- Bisulfite modification of DNA --- p.50 / Chapter 4.5.3 --- Methylation specific PCR (MSP) --- p.51 / Chapter 4.6 --- Microsatellite Analysis --- p.53 / Chapter 4.7 --- Statistical analysis --- p.56 / Chapter Chapter 5 --- Results / Chapter 5.1 --- Clinical-pathological features of endometrioid adenocarcinoma --- p.57 / Chapter 5.2 --- Promoter hypermethylation in endometrial cancer --- p.57 / Chapter 5.3 --- Microsatellite status (MSI) analysis --- p.65 / Chapter Chapter 6 --- Discussion / Chapter 6.1 --- Promoter hypermethylation in endometrial cancer --- p.71 / Chapter 6.1.1 --- Concurrent hypermethylation of multiple genesin endometrioid adenocarcinoma and its precursor lesions --- p.72 / Chapter 6.1.1.1 --- Promoter hypermethylation of E-cad --- p.73 / Chapter 6.1.1.2 --- Promoter hypermethylation of APC --- p.73 / Chapter 6.1.1.3 --- Promoter hypermethylation of MGMT --- p.74 / Chapter 6.1.1.4 --- Promoter hypermethylation of RASSF1A --- p.75 / Chapter 6.1.1.5 --- Promoter hypermethylation of hMLH-1 --- p.76 / Chapter 6.1.1.6 --- Promoter hypermethylation in ECA coexisting with hyperplasia and not coexisting with hyperplasia --- p.77 / Chapter 6.1.2 --- Promoter hypermethylation in SCA --- p.77 / Chapter 6.2 --- Microsatellite status analysis --- p.78 / Chapter 6.2.1 --- MSI in endometrial cancer --- p.78 / Chapter 6.2.2 --- MSI and concurrent promoter hypermethylation --- p.79 / Chapter 6.2.3 --- MSI and promoter hypermethylation of hMLH-1 --- p.80 / Chapter Chapter 7 --- Conclusion --- p.81 / Further studies --- p.82 / References --- p.83
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324734 |
| Date | January 2004 |
| Contributors | Cheung, Ka Wai., Chinese University of Hong Kong Graduate School. Division of Anatomical and Cellular Pathology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, ix, 93 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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