Chan Ling Nga. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 143-153). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgements --- p.vi / Table of Contents --- p.vii / List of Figures --- p.xi / List of Tables --- p.xiv / Abbreviations --- p.xv / Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- The Human Endometrium --- p.1 / Chapter 1.1.1 --- The Structure of the Endometrium --- p.1 / Chapter 1.1.2 --- Cyclic Changes in the Endometrium --- p.1 / Chapter 1.1.3 --- Physiological Roles of the Endometrium --- p.5 / Chapter 1.1.4 --- Roles of Luminal Epithelium in Implantation --- p.5 / Chapter 1.1.5 --- Exocrine Functions of the Endometrial Epithelium --- p.6 / Chapter 1.2 --- Review of Epithelial Ion Channels --- p.8 / Chapter 1.2.1 --- Epithelial Na+ Channels (ENaC) in Absorbing Epithelia --- p.9 / Chapter 1.2.2 --- Epithelial C1- Channels in Secretory Epithelia --- p.13 / Chapter 1.2.3 --- Na+ and C1- Channels in Endometrial Epithelia --- p.15 / Chapter 1.3 --- Review of the Intracellular Signal Transduction Pathways --- p.15 / Chapter 1.3.1 --- The cAMP-Mediated Signal Transduction Pathway --- p.17 / Chapter 1.3.2 --- The cAMP-Mediated Chloride Channels in Epithelial Cells --- p.17 / Chapter 1.3.3 --- Ca2+-Dependent Signal Transduction Pathway --- p.21 / Chapter 1.4 --- Physiological Roles of some Neurohormonal Agents in Uterine Functions: Selected Examples --- p.23 / Chapter 1.4.1 --- Roles of Adrenaline on the Endometrial Ion Transport --- p.23 / Chapter 1.4.2 --- Prostaglandin (PG) E2 and PGF2α --- p.24 / Chapter 1.4.3 --- Biological Effect of Extracellular Nucleotides --- p.26 / Chapter 1.5 --- Objective of this Study --- p.28 / Chapter 2 --- Materials and Methods --- p.31 / Chapter 2.1 --- Materials --- p.31 / Chapter 2.1.1 --- Culture Media and Enzymes --- p.31 / Chapter 2.1.2 --- Drugs --- p.31 / Chapter 2.1.3 --- Chemicals --- p.32 / Chapter 2.1.4 --- Experimental Tissues and Animals --- p.32 / Chapter 2.2 --- Preparations --- p.32 / Chapter 2.2.1 --- Previous Support for Cell Growth --- p.32 / Chapter 2.2.2 --- Growth Medium --- p.33 / Chapter 2.2.3 --- Culture of Mouse Endometrial Epithelial Cells --- p.35 / Chapter 2.2.4 --- Solutions for the Short-Circuit Current Measurements --- p.36 / Chapter 2.2.5 --- Solutions for the Patch-Clamp Experiments --- p.38 / Chapter 2.2.6 --- Running Buffers for RNA and DNA Gel Electrophoresis --- p.39 / Chapter 2.2.7 --- UTP-free UDP --- p.40 / Chapter 2.2.8 --- Electrodes for the Short-Circuit Current Measurement --- p.40 / Chapter 2.3 --- Protocols --- p.41 / Chapter 2.3.1 --- Characterization of Neurohormonal Agents-induced Ion Channels --- p.41 / Chapter 2.3.2 --- Possible Interaction between CFTR and ENaC --- p.41 / Chapter 2.3.3 --- Characterization of Pyrimidinoceptors-mediated Conductances --- p.42 / Chapter 2.4 --- Methods of Measurements --- p.42 / Chapter 2.4.1 --- The Patch-Clamp Technique --- p.42 / Chapter 2.4.1.1 --- The Patch-Clamp Setup --- p.43 / Chapter 2.4.1.2 --- Shielding and Grounding --- p.45 / Chapter 2.4.1.3 --- Pipette Fabrication --- p.45 / Chapter 2.4.1.4 --- Pipette Holder and Electrodes --- p.48 / Chapter 2.4.1.5 --- Experimental Procedures --- p.49 / Chapter 2.4.1.6 --- Signal Recording and Data Acquisition --- p.54 / Chapter 2.4.1.7 --- Data Analysis --- p.54 / Chapter 2.4.2 --- The Short-Circuit Current Technique --- p.55 / Chapter 2.4.2.1 --- The Short-Circuit Current Setup --- p.56 / Chapter 2.4.2.2 --- Experimental Procedures --- p.56 / Chapter 2.4.2.3 --- Data Analysis --- p.61 / Chapter 2.4.3 --- Reverse Transciption - Polymerase Chain Reaction (RT-PCR) --- p.61 / Chapter 2.4.3.1 --- RNA Isolation --- p.61 / Chapter 2.4.3.2 --- RNA Gel Electrophoresis --- p.62 / Chapter 2.4.3.3 --- Reverse Transcription (RT) --- p.63 / Chapter 2.4.3.4 --- Polymerase Chain Reaction (PCR) --- p.64 / Chapter 2.4.3.5 --- DNA Gel Electrophoresis --- p.66 / Chapter 2.4.4 --- Statistical Analysis --- p.66 / Chapter 3 --- Results --- p.67 / Chapter 3.1 --- Activation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Response to Hormonal Stimuli --- p.67 / Chapter 3.2 --- Inhibition of Na+ Absorption by CFTR --- p.89 / Chapter 3.3 --- Pyrimidinoceptors-activated Ca2+-dependent C1- Conductance --- p.111 / Chapter 4 --- General Discussions --- p.132 / Appendix --- p.140 / Chapter A --- RNA Isolation --- p.140 / Chapter B --- Reverse Transcription --- p.141 / Chapter C --- Polymerase Chain Reaction --- p.142 / References --- p.143
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322854 |
| Date | January 1999 |
| Contributors | Chan, Ling Nga., Chinese University of Hong Kong Graduate School. Division of Physiology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xvi, 153 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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