The Severe Acute Respiratory Syndrome (SARS) virus encodes several unusual
RNA processing enzymes, including Nsp15, an endoribonuclease that preferentially
cleaves 3? of uridylates through a Ribonuclease A-like mechanism. Crystal structures of
Nsp15 confirmed that the Nsp15 active site is structurally similar to that of Ribonuclease
A. These similarities and our molecular docking analysis lead us to hypothesize that
previously characterized Ribonuclease A inhibitors will also inhibit the SARS-CoV
Nsp15. Benzopurpurin B, C-467929, C-473872, N-36711, N-65828, N-103018 and
Congo red were tested for effects on Nsp15 endoribonuclease activity. A real-time
fluorescence assay revealed that the IC50 values for inhibiting Nsp15 were between 0.2
?M and 40 ?M. Benzopurpurin B, C-473872, and Congo red are competitive inhibitors,
according to kinetic studies and were demonstrated to bind SARS-CoV Nsp15 by a
differential scanning fluorimetry assay. Benzopurpurin B also inhibited the Nsp15
orthologs from two other coronaviruses: mouse hepatitis virus (MHV) and infectious
bronchitis virus. The three compounds reduced infectivity of MHV in L2 cells by 8 to 26
fold. The more effective drugs also caused a decrease in MHV RNA accumulation.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/ETD-TAMU-2009-05-307 |
| Date | 2009 May 1900 |
| Creators | Ortiz Alcantara, Joanna M. |
| Contributors | Kao, Cheng C. |
| Source Sets | Texas A and M University |
| Language | English |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | application/pdf |
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