Gel particle systems were studied as a means to separate macromolecules such as proteins and DNA. A mathematical model for diffusive - convective transport was developed in order to study the separation of two different components. The model used a one dimensional approximation for a gel particle in a packed column. The electrophoretic transport of a single solute species across a one dimensional three layered system was analyzed using the geometrical structure of the spectrum of the operator. The structure of the spectrum allows a complete characterization of all the eigenvalues of the system in terms of the various parameters including the distribution coefficients, electrical field, electrophoretic mobility, and diffusion coefficient. The separation of two components was parametrically studied by varying the relative ratio of Peclet numbers in the gel packed column. / A second aspect of protein separation examined in this project is adsorption effects in gel particle systems. An analysis using a Laplace transform was performed to investigate the separation behavior of gel chromatography. The ionic strength effects on adsorption were analyzed using the thermodynamic model extended from the work of Horvath (1976). / The adsorption of large DNA was found to be strongly influenced by ionic strength and gel surface area. Some experimental factors affecting DNA separation were discussed. / Source: Dissertation Abstracts International, Volume: 54-02, Section: B, page: 0972. / Major Professor: Bruce R. Locke. / Thesis (Ph.D.)--The Florida State University, 1993.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_76866 |
| Contributors | Park, Young Gyu., Florida State University |
| Source Sets | Florida State University |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | 245 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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