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Analýza low-copy sekvencí a její využití pro hodnocení polymorfismu kmenů/izolátů \kur{Beauveria bassiana} / Analysis of low-copy sequences and its utilization for evaluation of genetic polymorphism in selected \kur{Beauveria bassiana} strains/isolates

Beauveria bassiana is used in biological control against the economically significant pests. Detection of morphological and genetic polymorphism between different species and strains isolated in the natural environment is important to study the distribution and effects in the ecosystem. The aim of this study was to determine in which links are "local tribes" with ecological aspects of biological control in protected zone and assess the polymorphism of the sections by using molecular markers. Were analyzed 36 strains collected in the National Park Šumava in the Czech Republic. Polymorphism of these strains was determined according to a sequence of LSU (28S Large Subunit Ribosomal DNA). It is observed that the populations from National Park is closed, "the local tribes" because these methods can be very well characterized and compared with other strains from different parts of the Czech Republic and other countries. Populations from National Park showed no differences in polymorphism. Through this analysis, the sample Bba I101 was aligned. This preparation is re-used for bark beetle occurring in the National Park Šumava in the Czech Republic. This study was supported by grants GACR 521/08/H042, MSM 60076658-06, MGA SP/2d1/41/08.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:53173
Date January 2010
CreatorsJOZOVÁ, Eva
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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