The catalytic oxidation of bisphenol A (BPA) by laccase (EC 1.10.3.2) has recently attracted considerable attention because laccase can rapidly transform potentially persistent and toxic pollutants such as bisphenol A in the presence of molecular oxygen. Direct treatment of wastewaters containing bisphenol A would require excessive amounts of laccase if the enzyme is lost with the effluent and, thus, treatment would be cost-prohibitive. Therefore, strategies are desired for retaining the enzyme in the treatment system. It was envisioned that this could be accomplished by extracting pollutants into a non-aqueous phase liquid and then treating them in that phase using enzymatic catalysis. In such a treatment scheme, preserving the enzyme and maintaining its high reactivity in a non-aqueous phase liquid can be easily achieved through the use of reverse micelles. / Therefore, this study was conducted in order to examine the efficiency of enzymatic treatment of BPA in reverse micelles. It was found that laccase entrapped in the reverse micelles formed by dioctyl sulfosuccinate sodium salt (AOT) and isooctane exhibited the highest catalytic activity at a pH of 6, a water content of 10 (i.e., the molar ratio of water to AOT), and an AOT concentration of 80 mM.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.82614 |
| Date | January 2004 |
| Creators | Liu, Yan, 1975- |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Engineering (Department of Civil Engineering and Applied Mechanics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002211180, proquestno: AAIMR12628, Theses scanned by UMI/ProQuest. |
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