The optimization of the extraction and purification of horseradish peroxidase from horseradish roots

Description

Thesis (MSc)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: This study describes:
a) the optimization of the current industrial-scale extraction and purification of Horseradish
peroxidase from horseradish at BBI Enzymes, focussing on:
a. Raw material quality,
b. Extraction,
c. Ultra-filtration,
d. Salt fractionation,
e. Diafiltration,
f. Ion Exchange Chromatography,
b) developing an new in-process microtitre plate calorimetric assay,
c) characterization of main groups of HRP relevant to BBI Enzymes by SDS-PAGE- and HPLC
analysis. / AFRIKAANSE OPSOMMING: Hierdie studie beskryf:
a) die optimisering van die huidige industriële-skaal ekstraksie en suiwering van peperwortelperoksidase
vanuit peperwortel by BBI Enzymes, deur te focus op:
a. Rou material kwaliteit,
b. Ekstraksie,
c. Ultra-filtrasie,
d. Sout fraksionering,
e. Diafiltrasie,
f. Ioon-uitruilchromatografie
b) Ontwikkeling van ‘n nuwe in-proses mikro-titer gebaseerde kalorimetriese toetsmetode
c) die karakterisering van die hoof groepe peperwortel-peroksidase belangrik vir BBI Enzymes.

Links & Downloads

1. http://hdl.handle.net/10019.1/71784

Tags

Horseradish peroxidase

Enzymes -- Purification

Dissertations -- Biochemistry

Theses -- Biochemistry

Biochemistry

Additional Fields

Identifer	oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/71784
Date	12 1900
Creators	Barnard, Almero
Contributors	Swart, Pieter, Graz, Michael, Stellenbosch University. Faculty of Science. Dept. of Biochemistry.
Publisher	Stellenbosch : Stellenbosch University
Source Sets	South African National ETD Portal
Language	en_ZA
Detected Language	English
Type	Thesis
Format	132 p. : ill.
Rights	Stellenbosch University