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Cassette Systems for Creating Intergeneric Hybrid ATCases

Cassette systems for creating intergeneric hybrid ATCases were constructed. An MluI restriction enzyme site was introduced at the carbamoylphosphate binding site within the pyrB genes of both Pseudomonas putida and Escherichia coli. Two hybrids, E. coli pyrB polar domain fused with P. putida pyrB equatorial domain and P. putida pyrB polar domain fused with E. coli pyrB equatorial domain, are possible. The intergeneric E. coli-P. putida hybrid pyrB gene was constructed and found to encode an active ATCase which complemented an E. coli Pyr- strain. These hybrids are useful for kinetic and expression studies of ATCase in E. coli.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc2237
Date12 1900
CreatorsSimpson, Luci N.
ContributorsO'Donovan, Gerard A., Benjamin, Robert C., Farinha, Mark A.
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
FormatText
RightsPublic, Copyright, Simpson, Luci N., Copyright is held by the author, unless otherwise noted. All rights reserved.

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