This study explores relative levels of stxl and fur gene expression under antibiotic-stressed and control (non-stressed) Escherichia coli O157:H7 using real-time polymerase chain reaction (PCR) cycle threshold (CO value comparisons among replicates at designated time points of growth. Our data indicate that E. coli O157:H7 under the subinhibitory concentration(SIC) level of chloramphenicol decreases fur expression in early stationary phase cultures by 50% compared to non-stressed cells, but increases stxl expression by 35-50% during the log-to-stationary phase transition. Since the enterohemorrhagic E. coli stxl gene is negatively regulated by the fur gene product or results indicate that a separate fundamental transcriptional regulatory mechanism is functional in cultures grown under subinhibitory stress, such as antibiotic exposure. These data could support the clinical results obtained from treatment of EHEC-mediated toxicoinfections with antibiotics which have resulted inducing EHEC to prematurely produce cytotoxins within the host and speed the course of hemorrhagic colitis (HC) and/or hemolytic uremic syndrome (HUS). / Department of Biology
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/188256 |
| Date | January 2007 |
| Creators | Charkhezarrin, Samila |
| Contributors | McKillip, John L. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | viii, 64 leaves : ill. (some col.) ; 28 cm. |
| Source | Virtual Press |
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