Soils contain highly diverse consortia of bacteria making them very attractive starting points for both culture-dependent and metagenomic discovery efforts. The present diploma thesis analyses the composition of the microbial community from pharmaceutically polluted soil, with the employment of next-generation Illumina sequencing of 16S rDNA region. This analysis revealed high complexity of the soil microbial environment and confirmed that anthropogenic activity (represented by production of beta- lactam antibiotics) influences the variability and abundance of the species, yet without reducing the microbial diversity. In the second part of the thesis, isolation and heterologous expression of a novel gene encoding alpha-amino acid ester hydrolase (AEH) from a cultivable soil microorganism B. cereus is described. AEHs possess industrial potential for biocatalytic synthesis of semi-synthetic beta-lactam antibiotics, which are presently of great clinical importance. Powered by TCPDF (www.tcpdf.org)
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:331139 |
| Date | January 2015 |
| Creators | Pitkina, Anastasiya |
| Contributors | Kyslík, Pavel, Lichá, Irena |
| Source Sets | Czech ETDs |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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