The topic of this work was the use of variable DNA regions for clarification of phylogenic relations of closely related South African species of Crassula L. genus. Based on preliminary dendrogram which was constructed according to chloroplast regions, 69 members of Crassula genus were used for analysis. Variable regions of chloroplast DNA (matK, rps16) as well as nuclear regions of DNA (ITS, ETS) of these members were analyzed. This work was focused on variable regions of nuclear DNA mainly to ETS region which was found as the most suita-ble for phylogenic study of this group of plants. These four regions of DNA were amplified and sequenced for all 69 analyzed members of Crassula genus. The multiple alignments of all DNA sequences were evaluated. The results confirm hypothesis that chosen noncoding nucle-ar DNA regions can be more suitable for phylogenetic study of these closely related species than chloroplast DNA regions because of their much higher variability. The ETS region shoved highest variability of all regions used in this work, so it appears as suitable for detailed phylogenetic analysis of the Crassula genus.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:426882 |
| Date | January 2018 |
| Creators | Jánová, Anna |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
Page generated in 0.002 seconds