The in vivo 3D extracellular matrix provides a temporal regulatory environment
of chemical cues. Understanding this dynamic environment will be crucial for efficient
drug screening, diseases mechanism elucidation, and tissue engineering. Therefore, in
vitro 3D cell culture systems with reversible chemical environments are required. To this
end, we developed a non-cytotoxic agarose-desthiobiotin hydrogel to sequester
streptavidin biomolecule conjugates (KD 10-11 M), which can then be displaced by the
addition of biotin (KD 10-15 M). Streptavidin biomolecule conjugates were simultaneously
and sequentially immobilized by changing media components. The time required for
biochemical environment exchange was minimized by increasing the surface area to
volume ratios and pore size of the hydrogels. We temporally controlled the cell adhesive
properties of hydrogels with RGD modified streptavidin to influence endothelial cell tube
formation. / Thesis / Master of Science (MSc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/22743 |
| Date | January 2018 |
| Creators | Nijsure, Devang |
| Contributors | Wylie, Ryan G., Chemical Biology |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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