Microtubules play a major role in intracellular trafficking of vesicles in endocrine cells. Detailed knowledge of microtubule organization and their relation to other cell constituents is crucial for understanding cell function. However, their role in insulin transport and secretion is currently under debate. Here, we use Fib-Sem to image islet beta cells in their entirety with unprecedented resolution. We reconstruct mitochondria, Golgi apparati, centrioles, insulin secretory granules and micro-tubules of seven beta cells, and generate a comprehensive spatial map of microtubule-organelle interactions. We find that micro-tubules form non-radial networks that are predominantly not connected to either centrioles or endomembranes. Microtubule number and length, but not microtubule polymer density, vary with glucose stimulation. Furthermore, insulin secretory granules are enriched near the plasma membrane where they associate with microtubules. In summary, we provide the first 3D reconstructions of complete microtubule networks in primary mammalian cells together with evidence regarding their importance for insulin secretory granule positioning and thus supportive role in insulin secretion.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:73356 |
| Date | 19 January 2021 |
| Creators | Müller, Andreas, Schmidt, Deborah, Xu, C. Shan, Pang, Song, D'Costa, Jolson Verner, Kretschmar, Susanne, Münster, Carla, Kurth, Thomas, Jug, Florian, Weigert, Martin, Hess, Harald F., Solimena, Michele |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 10.1101/2020.10.07.329268, info:eu-repo/grantAgreement/European Commission/H2020 | IMI2-RIA/115881//Assessing risk and progression of prediabetes and type 2 diabetes to enable disease modification/RHAPSODY, info:eu-repo/grantAgreement/European Commission/H2020 | IMI2-RIA/115797//Translational approaches to disease modifying therapy of type 1 diabetes: an innovative approach towards understanding and arresting type 1 diabetes/INNODIA |
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