In the first part of this study, the effect of diabetes mellitus on the long bones (femur and tibia) of the streptozocin induced diabetic rats and the effect of selenium (Se) treatment on these bones are investigated at molecular level by Fourier transform infrared (FTIR) spectroscopy, light and electron microscopy. In the second part of this study, the effect of selenium and vitamin E deficiency or selenium toxicity on rat bones have been studied by FTIR spectroscopy.
The results of the first part of the present study revealed that the changes observed in the mineral and matrix phases of diabetic bones, briefly, the increase in the mineral crystal size, the decrease in the acid phosphate and carbonate content, the increase in the ratio of pyridinoline [Pyr] cross-links to dihydroxylysinonorleucine [DHLNL] cross-links present in collagen I of the bone tissue as well as the increase in the lipid to protein ratio of the matrix are quite similar to those seen in osteoporotic patients and animal models and confirms the evidence of diabetic osteoporosis. Histologic studies carried out with light and electron microscopy supported these findings. FTIR spectroscopic analysis revealed that sodium selenite treatment had some restoring effects on the deviated properties of the microstructure of diabetic bones.
The results of the second part of this study revealed that the deficiency of selenium led to increase in the crystal size of the bone minerals, decreases in acid phosphate and labile carbonate content and increase in the Pyr to DHLNL ratio as in the case of diabetic bones. These results can be indicative of the importance of selenium in glucose metabolism.The results of Se excess group are similar to those of Se deficient group except that toxic amount of selenium led to increase in the relative amount of acid phosphate. This can affect the pH of the mineral environment and lead to deformation of the bone tissue. It can be concluded from FTIR spectroscopic and light microscopic findings that both antioxidant deficient and excess diets cause almost similar defects in the mineral matrix phases of rat long bones.
Overall results may reflect the importance of antioxidants for human life and if they are used in proper amounts they can be preventive for the complications of diabetes seen in bones as well as other organs. However, further investigations are necessary for the therapeutic usage of selenium, since the treatment of control group rat femurs with sodium selenite led to some structural defects.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12605038/index.pdf |
| Date | 01 June 2004 |
| Creators | Boyar, Handan |
| Contributors | Severcan, Feride |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | Ph.D. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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