Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Zdeňka Čermáková Supervisor: Prof. RNDr. Jiří Lamka, CSc. Title of diploma thesis: The progression and results of the model parasite culturing liver fluke (Fasciola hepatica) in sheep (Ovis aries) Fasciolosis is a parasitic disease caused by liver fluke (Fasciola hepatica). This parasitosis is a significant disease in livestock, wild animals and in some parts of the world in humans. It causes a decrease in livestock performance and fertility, weight loss and high economic losses in livestock. The aim of this work was to describe in detail the course of artificial infection of sheep infected with F. hepatica metacercaria. Specifically, changes in blood count, egg excretion, and response of the immune system of infected individuals were monitored. The observed values were compared with the studies already performed. The demonstration of fasciolosis in artificially infected sheep was performed using the coprological sedimentation method, the differential determination of leukocytes from the blood layers, the determination of hematocrit and the immunological method ELISA (enzyme-linked immunosorbent assays). Three lambs of Texel breeds infected with 200 metacercaria were used for the...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:382757 |
| Date | January 2018 |
| Creators | Čermáková, Zdeňka |
| Contributors | Lamka, Jiří, Jílek, Petr |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
Page generated in 0.0067 seconds