Cyclopropenoid fatty acids cause several physiological disorders
in rainbow trout and rats. Rainbow trout appear to be more sensitive
than rats to the effects of cyclopropenoid fatty acids. Carbon-14
labeled sterculic acid with label in the methylene position of the
cyclopropene ring was injected into the stomach of rats and rainbow
trout in order to obtain information on the metabolism of sterculic
acid in both species. Data were gathered on deposition of carbon-14
label in tissue, distribution of label in liver subcellular fractions
and excretion of label in urine, feces and CO₂. Metabolites in excreta
were isolated by gas liquid chromatography and identified by infrared
spectroscopy, mass spectroscopy and nuclear magnetic resonance.
The liver was the organ with the highest radioactivity with peak
concentrations of 11% of the administered dose in rat liver after
four hours and 2.8% in rainbow trout liver after three and five days.
Liver microsomal fractions contained 1.1% of the administered dose at
peak liver concentrations in both species.
Rainbow trout excreted C¹⁴ label at a much slower rate than the
rat. Rats eliminated 48% in the urine and 11% in feces after 16 hours. Rainbow trout eliminated 50% of the administered dose after five days.
A significant amount of enterohepatic circulation was indicated in the
rainbow trout, since at five days at least 25% of the carbon-14 label
injected was found in blood plasma, upper intestine, pyloric ceacum
and liver. A maximum of 1% of the label was found in expired CO₂ of
the rat and 0.5% in the expired CO₂ of the rainbow trout, suggesting
that the cyclopropene ring cannot be metabolized to CO₂
Cis and trans-3,4-methylene adipic acid and cis-3,4-methylene
suberic acid were isolated from rat urine. Sterculic acid would have
to undergo beta and omega oxidation plus reduction of the cyclopropene
ring to form the metabolites. Cis-3,4-methylene adipic acid was the
major metabolite. Metabolites in the rainbow trout excreta could
not be identified. However, gas liquid chromatography data indicated
rainbow trout metabolites were not the same as those isolated from
rat excreta. / Graduation date: 1977
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27099 |
| Date | 01 June 1976 |
| Creators | Yoss, James Kenneth |
| Contributors | Nixon, Joseph E. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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