Bisphenols are used in the process of polymerization of polycarbonate plastics and epoxy
resins. Bisphenols can easily migrate out of plastic products and enter the gastrointestinal system.
By increasing colonic inflammation in mice, disrupting the intestinal bacterial community structure
and altering the microbial membrane transport system in zebrafish, bisphenols seem to interfere with
the gut microbiome. The highly abundant human commensal bacterium Bacteroides thetaiotaomicron
was exposed to bisphenols (Bisphenol A (BPA), Bisphenol F (BPF), Bisphenol S (BPS)), to examine
the mode of action, in particular of BPF. All chemicals caused a concentration-dependent growth
inhibition and the half-maximal effective concentration (EC50) corresponded to their individual logP
values, a measure of their hydrophobicity. B. thetaiotaomicron exposed to BPF decreased membrane
fluidity with increasing BPF concentrations. Physiological changes including an increase of acetate
concentrations were observed. On the proteome level, a higher abundance of several ATP synthase
subunits and multidrug efflux pumps suggested an increased energy demand for adaptive mechanisms after BPF exposure. Defense mechanisms were also implicated by a pathway analysis that
identified a higher abundance of members of resistance pathways/strategies to cope with xenobiotics (i.e., antibiotics). Here, we present further insights into the mode of action of bisphenols in a
human commensal gut bacterium regarding growth inhibition, and the physiological and functional
state of the cell. These results, combined with microbiota-directed effects, could lead to a better
understanding of host health disturbances and disease development based on xenobiotic uptake.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:88407 |
| Date | 01 December 2023 |
| Creators | Riesbeck, Sarah, Petruschke, Hannes, Rolle-Kampczyk, Ulrike, Schori, Christian, H. Ahrens, Christian, Eberlein, Christian, J. Heipieper, Hermann, von Bergen, Martin, Jehmlich, Nico |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2076-2607, 10.3390/microorganisms10081610 |
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