Diabetes mellitus has emerged as a major public health problem with pandemic
growth as the International Diabetes Federation estimates that there were 415
million diabetics in 2015 with that number reaching 642 million by 2040, affecting
all regions of the world. Globally we are all interconnected when we deal with
problems of climate change, water shortage, HIV or Ebola. The war against type 2
diabetes and other non-communicable diseases should be no different, as
effective solutions will need expanded global engagement in science to win it.
The risk of cardiovascular events in type 2 diabetes remains unchanged despite
good control of diabetes and other cardiovascular risk factors. A better
understanding of thrombogenicity in diabetes may help to identify novel
therapeutic agents and a starting point would be to identify ultrastructural changes
in diabetic erythrocytes, platelets and fibrin networks. In diabetes, thrombogenicity
is enhanced and is characterised by: hyperactive platelets, higher levels of clotting
factors and impaired fibrinolysis. Thus, in this research study, the technique of
scanning electron microscopy (SEM) was used to identify ultrastructural
abnormalities in erythrocytes, platelets and fibrin networks of diabetic subjects.
Distinct abnormal morphological findings were observed in the erythrocytes,
platelets and fibrin fibres of diabetic subjects in comparison to the controls.
Physiological parameters such as platelet markers and tissue factor levels were
also assessed. Flow cytometric analysis revealed hyperactive platelets in the
diabetic subjects. The measurement of tissue factor in plasma was completed by
using an ELISA. Tissue factor levels in the diabetic subjects were markedly
elevated when compared to controls.
Biomedical research has provided evidence that has led to the hypothesis that
inflammation is the culprit behind almost most chronic illnesses. Hyperglycaemia,
a key feature of diabetes, is known to promote a state of low-grade chronic
inflammation. A natural method that can resolve acute and chronic inflammation is
earthing. Earthing involves coupling your body to the Earth's surface energies by simply walking barefoot or being connected to a conductive device. When earthed,
the electrons are conducted into the human body at the same electrical potential
as the earth. It is also suggested that free electrons from the earth neutralize the
positively charged free radicals that are the hallmark of chronic inflammation. In
this study, earthing was accomplished with conductive adhesive patches placed
on the sole of each foot and palm of each hand. An earthing cord was connected
to the patches and led outdoors to be connected to a stainless-steel rod driven into
the ground. Diabetic subjects were earthed for a session of two hours. Bloods
were drawn before and just prior to the end of the two-hour session. Morphological
SEM findings of the erythrocytes, platelets and fibrin networks at two-hours
showed a remarkable difference when compared to findings at baseline. More
importantly, the erythrocytes, platelets and fibrin findings revealed that they all
almost reverted to looking like control erythrocytes, platelets and fibrin networks. It
remains to be seen if earthing will reduce cardiovascular events in diabetics by
improving morphology of cells involved in coagulation. / Thesis (PhD)--University of Pretoria, 2016. / Physiology / PhD / Unrestricted
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:up/oai:repository.up.ac.za:2263/61669 |
| Date | January 2016 |
| Creators | Soma, Prashilla |
| Contributors | Pretorius, Etheresia, PSOMA@MED.UP.AC.ZA |
| Publisher | University of Pretoria |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | © 2017 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
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