The purpose of this study was to investigate whether
the beneficial effects which the consumption of fish oil
imparts on hemostatic function can be modified by the level
of dietary selenium. Male Sprague Dawley rats were fed for
eight weeks semipurified diets containing 7% corn oil (by
weight) or 5.5% fish oil (MaxEPA) plus 1.5% corn oil with or
without selenium supplementation.
The indicators of selenium status (glutathione
peroxidase activity and selenium level) were significantly
lower in the rats given inadequate selenium, regardless of
the type of fat fed. In the animals fed adequate selenium,
these same indicators tended to be lower when fish oil was
fed. Although feeding of fish oil increased hepatic and
aortic malondialdehyde (MDA), selenium supplementation
decreased its level in the liver.
Selenium deficiency led to a decrease in the relative
weight percent of 22:6 n-3 in aorta and plasma. Increases in the levels of 20:5 n-3, 22:5 n-3, 22:6 n-3, 20:3 n-6 and
a decrease in the level of 20:4 n-6 were observed in plasma
total lipids and aortic and hepatic phospholipids when fish
oil was fed. The increased level of 20:3 n-6 suggests that
delta 5-desaturase activity was decreased by fish oil
feeding.
The level of aortic 6-keto-prostaglandin F₁alpha (6-
keto-PGF₁alpha) was highest in the rats fed diets that
contained corn oil but no selenium supplementation; selenium
supplementation, however, eliminated the difference in the
level of 6-keto-PGF₁alpha between fish oil and corn oil fed
groups. The levels of thromboxane B₂ (TXB₂) and ADP-induced
platelet aggregation were decreased significantly by fish
oil feeding and tended to be lower with selenium
supplementation. Selenium supplementation did not increase
bleeding time while fish oil feeding did.
These data indicate that selenium supplementation may
decrease fish oil induced lipid peroxidation in liver: this
is reflected in the lower hepatic MDA levels in the fish oil
fed animals with selenium supplementation and the increased
22:6 n-3 levels in aorta and plasma. Selenium deficiency
led to an increase in the level of 6-keto-PGF₁alpha in the
rats fed corn oil. Its effects on TXB₂ level and ADP-induced
platelet aggregation are marginal. Overall the
beneficial effect of selenium supplementation on hemostatic
function appears weaker than that of fish oil feeding. / Graduation date: 1990
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27068 |
| Date | 12 October 1989 |
| Creators | Song, Jihyun |
| Contributors | Wander, Rosemary C. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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