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Molecular cloning and characterisation of a putative peroxidase cDNA from flax (Linum usitatissimum L.)

The corresponding isoperoxidases from the flax genotrophs L and S have different molecular weights. Since further structural analysis will rely on knowledge of the primary structures of these molecules, the isolation of a molecular clone of this enzyme was attempted. Probing a cDNA library with a synthetic oligonucleotide was not successful, but a partial cDNA sequence was isolated by probing an S genotroph stem $ lambda$gt11 library with polyclonal antibodies raised against anionic plant peroxidases. Its longest reading frame sequence is divergent from those of other peroxidases, but contains some related segments. Genomic Southern analysis of the clone is presented, along with a study of the expression of its corresponding mRNA in plant tissues. A study on the effects of IAA, ABA and kinetin levels on the expression of peroxidase and of the putative peroxidase cloned mRNA is also presented.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59299
Date January 1989
CreatorsBeaulieu, Normand
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageMaster of Science (Department of Biology.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001067559, proquestno: AAIMM63513, Theses scanned by UMI/ProQuest.

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