Sickle cell disease is an inherited blood cell disorder that affects about 100,000 people
in the US and results in high cost of medical care exceeding $1.1 billion annually. Sickle
cell patients suffer from unpredictable, painful vaso-occlusive crises. Portable, costeffective
approaches for diagnosis and monitoring sickle blood activities are important for
a better management of the disease and reducing the medical cost.
In this research, a mobile application controlled, impedance-based flow cytometer is
developed for the diagnosis of sickle cell disease. Calibration of the portable device is
performed using a component of known impedance value. The preliminary test results are
then compared to those obtained by a commercial benchtop impedance analyzer for further
validation. With the developed portable flow cytometer, experiments are performed on two
sickle cell samples and a healthy cell sample. The acquired results are subsequently
analyzed with MATLAB scripts to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals
are observed between sickle cells and normal cells, as well as between sickle cells under
hypoxia and normoxia conditions. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2018. / FAU Electronic Theses and Dissertations Collection
| Identifer | oai:union.ndltd.org:fau.edu/oai:fau.digital.flvc.org:fau_40919 |
| Contributors | Dieujuste, Darryl (author), Zhuang, Hanqi (Thesis advisor), Du, Sarah (Thesis advisor), Florida Atlantic University (Degree grantor), College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science |
| Publisher | Florida Atlantic University |
| Source Sets | Florida Atlantic University |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation, Text |
| Format | 57 p., application/pdf |
| Rights | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder., http://rightsstatements.org/vocab/InC/1.0/ |
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